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Revealing the inventory of type III effectors in Pantoea agglomerans gall‐forming pathovars using draft genome sequences and a machine‐learning approach
Year:
2018
Source of publication :
Molecular Plant Pathology
Authors :
Chalupowicz, Laura
;
.
Manulis-Sasson, Shulamit
;
.
Morozov, Michael
;
.
Nissan, Gal
;
.
Volume :
19(2)
Co-Authors:

Michael Gershovits, Tal Pupko - Department of Cell Research and Immunology, Faculty of Life Sciences, Tel‐Aviv University, Tel‐Aviv

Guido Sessa, Isaac Barash - Department of Molecular Biology and Ecology of Plants, Faculty of Life Sciences, Tel‐Aviv University, Tel‐Aviv,

 

Facilitators :
From page:
381
To page:
392
(
Total pages:
12
)
Abstract:

Pantoea agglomerans, a widespread epiphytic bacterium, has evolved into a hypersensitive response and pathogenicity (hrp)‐dependent and host‐specific gall‐forming pathogen by the acquisition of a pathogenicity plasmid containing a type III secretion system (T3SS) and its effectors (T3Es). Pantoea agglomerans pv. betae (Pab) elicits galls on beet (Beta vulgaris) and gypsophila (Gypsophila paniculata), whereas P. agglomerans pv. gypsophilae (Pag) incites galls on gypsophila and a hypersensitive response (HR) on beet. Draft genome sequences were generated and employed in combination with a machine‐learning approach and a translocation assay into beet roots to identify the pools of T3Es in the two pathovars. The genomes of the sequenced Pab4188 and Pag824‐1 strains have a similar size (∼5 MB) and GC content (∼55%). Mutational analysis revealed that, in Pab4188, eight T3Es (HsvB, HsvG, PseB, DspA/E, HopAY1, HopX2, HopAF1 and HrpK) contribute to pathogenicity on beet and gypsophila. In Pag824‐1, nine T3Es (HsvG, HsvB, PthG, DspA/E, HopAY1, HopD1, HopX2, HopAF1 and HrpK) contribute to pathogenicity on gypsophila, whereas the PthG effector triggers HR on beet. HsvB, HsvG, PthG and PseB appear to endow pathovar specificities to Pab and Pag, and no homologous T3Es were identified for these proteins in other phytopathogenic bacteria. Conversely, the remaining T3Es contribute to the virulence of both pathovars, and homologous T3Es were found in other phytopathogenic bacteria. Remarkably, HsvG and HsvB, which act as host‐specific transcription factors, displayed the largest contribution to disease development.

Note:
Related Files :
galls
Gypsophila
Pantoea agglomerans pv. betae
Pantoea agglomerans pv. gypsophilae
pathoadaptive
pseB
table beet
Show More
Related Content
More details
DOI :
10.1111/mpp.12528
Article number:
0
Affiliations:
Database:
PubMed
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
44117
Last updated date:
02/03/2022 17:27
Creation date:
06/10/2019 10:38
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Scientific Publication
Revealing the inventory of type III effectors in Pantoea agglomerans gall‐forming pathovars using draft genome sequences and a machine‐learning approach
19(2)

Michael Gershovits, Tal Pupko - Department of Cell Research and Immunology, Faculty of Life Sciences, Tel‐Aviv University, Tel‐Aviv

Guido Sessa, Isaac Barash - Department of Molecular Biology and Ecology of Plants, Faculty of Life Sciences, Tel‐Aviv University, Tel‐Aviv,

 

Revealing the inventory of type III effectors in Pantoea agglomerans gall‐forming pathovars using draft genome sequences and a machine‐learning approach

Pantoea agglomerans, a widespread epiphytic bacterium, has evolved into a hypersensitive response and pathogenicity (hrp)‐dependent and host‐specific gall‐forming pathogen by the acquisition of a pathogenicity plasmid containing a type III secretion system (T3SS) and its effectors (T3Es). Pantoea agglomerans pv. betae (Pab) elicits galls on beet (Beta vulgaris) and gypsophila (Gypsophila paniculata), whereas P. agglomerans pv. gypsophilae (Pag) incites galls on gypsophila and a hypersensitive response (HR) on beet. Draft genome sequences were generated and employed in combination with a machine‐learning approach and a translocation assay into beet roots to identify the pools of T3Es in the two pathovars. The genomes of the sequenced Pab4188 and Pag824‐1 strains have a similar size (∼5 MB) and GC content (∼55%). Mutational analysis revealed that, in Pab4188, eight T3Es (HsvB, HsvG, PseB, DspA/E, HopAY1, HopX2, HopAF1 and HrpK) contribute to pathogenicity on beet and gypsophila. In Pag824‐1, nine T3Es (HsvG, HsvB, PthG, DspA/E, HopAY1, HopD1, HopX2, HopAF1 and HrpK) contribute to pathogenicity on gypsophila, whereas the PthG effector triggers HR on beet. HsvB, HsvG, PthG and PseB appear to endow pathovar specificities to Pab and Pag, and no homologous T3Es were identified for these proteins in other phytopathogenic bacteria. Conversely, the remaining T3Es contribute to the virulence of both pathovars, and homologous T3Es were found in other phytopathogenic bacteria. Remarkably, HsvG and HsvB, which act as host‐specific transcription factors, displayed the largest contribution to disease development.

Scientific Publication
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