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The Role of Carotenogenic Metabolic Flux in Carotenoid Accumulation and Chromoplast Differentiation: Lessons From the Melon Fruit
Year:
2019
Source of publication :
Frontiers in Plant Science
Authors :
Baumkoler, Fabian
;
.
Burger, Joseph
;
.
Feder, Ari
;
.
Freiman, Zohar E.
;
.
Gal-On, Amit
;
.
Gur, Amit
;
.
Katzir, Nurit
;
.
Meir, Ayala
;
.
Ohali, Shachar
;
.
Sa'ar, Uzi
;
.
Schaffer, Arthur
;
.
Shnaider, Yula
;
.
Tadmor, Yaakov
;
.
Tzuri, Galil
;
.
Wolf, Dalia
;
.
Volume :
10
Co-Authors:

Noam Chayut - Germplasm Resource Unit, John Innes Center, Norwich, United Kingdom

Li Li - Robert W. Holley Center for Agriculture and Health, USDA-ARS, Cornell University, Ithaca, NY, United States

Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:

Carotenoids have various roles in plant physiology. Plant carotenoids are synthesized in plastids and are highly abundant in the chromoplasts of ripening fleshy fruits. Considerable research efforts have been devoted to elucidating mechanisms that regulate carotenoid biosynthesis, yet, little is known about the mechanism that triggers storage capacity, mainly through chromoplast differentiation. The Orange gene (OR) product stabilizes phytoene synthase protein (PSY) and triggers chromoplast differentiation. OR underlies carotenoid accumulation in orange cauliflower and melon. The OR’s ‘golden SNP’, found in melon, alters the highly evolutionary conserved Arginine108 to Histidine and controls β-carotene accumulation in melon fruit, in a mechanism yet to be elucidated. We have recently shown that similar carotenogenic metabolic flux is active in non-orange and orange melon fruit. This flux probably leads to carotenoid turnover but known carotenoid turnover products are not detected in non-orange fruit. Arrest of this metabolic flux, using chemical inhibitors or mutations, induces carotenoid accumulation and biogenesis of chromoplasts, regardless of the allelic state of OR. We suggest that the ‘golden SNP’ induces β-carotene accumulation probably by negatively affecting the capacity to synthesize downstream compounds. The accumulation of carotenoids induces chromoplast biogenesis through a metabolite-induced mechanism. Carotenogenic turnover flux can occur in non-photosynthetic tissues, which do not accumulate carotenoids. Arrest of this flux by the ‘golden SNP’ or other flux-arrest mutations is a potential tool for the biofortification of agricultural products with carotenoids.

Note:
Related Files :
Carotenoid accumulation
carotenoids
Cucumis melo
Melon
metabolic flux
OR genes
Solanum lycopersicum
tomato
Show More
Related Content
More details
DOI :
10.3389/fpls.2019.01250
Article number:
1250
Affiliations:
Database:
PubMed
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
45715
Last updated date:
02/03/2022 17:27
Creation date:
31/12/2019 11:14
Scientific Publication
The Role of Carotenogenic Metabolic Flux in Carotenoid Accumulation and Chromoplast Differentiation: Lessons From the Melon Fruit
10

Noam Chayut - Germplasm Resource Unit, John Innes Center, Norwich, United Kingdom

Li Li - Robert W. Holley Center for Agriculture and Health, USDA-ARS, Cornell University, Ithaca, NY, United States

The Role of Carotenogenic Metabolic Flux in Carotenoid Accumulation and Chromoplast Differentiation: Lessons From the Melon Fruit

Carotenoids have various roles in plant physiology. Plant carotenoids are synthesized in plastids and are highly abundant in the chromoplasts of ripening fleshy fruits. Considerable research efforts have been devoted to elucidating mechanisms that regulate carotenoid biosynthesis, yet, little is known about the mechanism that triggers storage capacity, mainly through chromoplast differentiation. The Orange gene (OR) product stabilizes phytoene synthase protein (PSY) and triggers chromoplast differentiation. OR underlies carotenoid accumulation in orange cauliflower and melon. The OR’s ‘golden SNP’, found in melon, alters the highly evolutionary conserved Arginine108 to Histidine and controls β-carotene accumulation in melon fruit, in a mechanism yet to be elucidated. We have recently shown that similar carotenogenic metabolic flux is active in non-orange and orange melon fruit. This flux probably leads to carotenoid turnover but known carotenoid turnover products are not detected in non-orange fruit. Arrest of this metabolic flux, using chemical inhibitors or mutations, induces carotenoid accumulation and biogenesis of chromoplasts, regardless of the allelic state of OR. We suggest that the ‘golden SNP’ induces β-carotene accumulation probably by negatively affecting the capacity to synthesize downstream compounds. The accumulation of carotenoids induces chromoplast biogenesis through a metabolite-induced mechanism. Carotenogenic turnover flux can occur in non-photosynthetic tissues, which do not accumulate carotenoids. Arrest of this flux by the ‘golden SNP’ or other flux-arrest mutations is a potential tool for the biofortification of agricultural products with carotenoids.

Scientific Publication
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