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The wild metabolism of domesticated tomato – multi-omic analysis of a Solanum lycopersicum-pennellii introgression population
Year:
2020
Source of publication :
Authors :
Bocobza, Samuel
;
.
Volume :
Co-Authors:

J. Szymanski - Leibniz Institute of Plant Genetics and Crop Plant Research, Department of Molecular Genetics, Gatersleben, Germany
S. Panda - Weizmann Institute of Science, Department of Environmental and Plant Sciences, Rehovot, Israel
P. Sonawane - Weizmann Institute of Science, Department of Environmental and Plant Sciences, Rehovot, Israel
P. Cardenas - University of Copenhagen, Department of Plant and Environmental Sciences, Copenhgen, Denmark
J. Lashbrooke - Stellenbosch University, Faculty of AgriSciences, Stellenbosch, South Africa
A. Kamble - Savirtibai Phule Pune University, Department of Botany, Pune, India
N. Shachaf - Weizmann Institute of Science, Department of Environmental and Plant Sciences, Rehovot, Israel
A. Bovy - Wageningen Plant Research, Department of Plant Breeding, Wageningen, Netherlands
J. Beekwilder - Wageningen Plant Research, Department of Plant Breeding, Wageningen, Netherlands
Y. Tikunov - Wageningen Plant Research, Department of Plant Breeding, Wageningen, Netherlands
I. Romero de la Fuente - Wageningen Plant Research, Department of Plant Breeding, Wageningen, Netherlands
D. Zamir -
The Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot, Israel
I. Rogachev - Weizmann Institute of Science, Department of Environmental and Plant Sciences, Rehovot, Israel
A. Aharoni - Weizmann Institute of Science, Department of Environmental and Plant Sciences, Rehovot, Israel

 

Facilitators :
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To page:
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Total pages:
1
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Abstract:

Wild tomato species represent a rich gene pool for numerous desirable traits lost during domestication. Many of these traits are related to the ripening dynamics of secondary metabolism, resulting in modified accumulation of defence-related and antinutritional compounds. Here, we exploited an introgression population developed from the wild desert-adapted tomato Solanum pennellii and the domesticated cultivar Solanum lycopersicum to identify the genetic basis of fruit metabolic variation accompanying wild species’ trait transfer. RNAseq profiling and LC-qTOF-MS-based metabolomics of 580 lines coupled to pathogen sensitivity assays resulted in the identification of genetic loci significantly associated with levels of hundreds of transcripts and metabolites. These associations occurred in functionally coherent hotspots that represent coordinated perturbation of metabolic pathways and ripening-related processes. The approach successfully highlighted new enzymes of the steroidal glycoalkaloid biosynthetic pathway, which we validated here experimentally. Finally, we showed that fruit resistance to Botrytis cinerea is closely associated with specific changes in the secondary metabolism and the fruit ripening regulatory network.

Note:
Related Files :
Data integration
fruit ripening
Metabolomics
pathogen defence
systems biology
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DOI :
Article number:
0
Affiliations:
Database:
Google Scholar
Publication Type:
Abstract
;
.
Conference paper
;
.
Language:
English
Editors' remarks:
ID:
46072
Last updated date:
02/03/2022 17:27
Creation date:
09/02/2020 15:23
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Scientific Publication
The wild metabolism of domesticated tomato – multi-omic analysis of a Solanum lycopersicum-pennellii introgression population

J. Szymanski - Leibniz Institute of Plant Genetics and Crop Plant Research, Department of Molecular Genetics, Gatersleben, Germany
S. Panda - Weizmann Institute of Science, Department of Environmental and Plant Sciences, Rehovot, Israel
P. Sonawane - Weizmann Institute of Science, Department of Environmental and Plant Sciences, Rehovot, Israel
P. Cardenas - University of Copenhagen, Department of Plant and Environmental Sciences, Copenhgen, Denmark
J. Lashbrooke - Stellenbosch University, Faculty of AgriSciences, Stellenbosch, South Africa
A. Kamble - Savirtibai Phule Pune University, Department of Botany, Pune, India
N. Shachaf - Weizmann Institute of Science, Department of Environmental and Plant Sciences, Rehovot, Israel
A. Bovy - Wageningen Plant Research, Department of Plant Breeding, Wageningen, Netherlands
J. Beekwilder - Wageningen Plant Research, Department of Plant Breeding, Wageningen, Netherlands
Y. Tikunov - Wageningen Plant Research, Department of Plant Breeding, Wageningen, Netherlands
I. Romero de la Fuente - Wageningen Plant Research, Department of Plant Breeding, Wageningen, Netherlands
D. Zamir -
The Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot, Israel
I. Rogachev - Weizmann Institute of Science, Department of Environmental and Plant Sciences, Rehovot, Israel
A. Aharoni - Weizmann Institute of Science, Department of Environmental and Plant Sciences, Rehovot, Israel

 

Wild tomato species represent a rich gene pool for numerous desirable traits lost during domestication. Many of these traits are related to the ripening dynamics of secondary metabolism, resulting in modified accumulation of defence-related and antinutritional compounds. Here, we exploited an introgression population developed from the wild desert-adapted tomato Solanum pennellii and the domesticated cultivar Solanum lycopersicum to identify the genetic basis of fruit metabolic variation accompanying wild species’ trait transfer. RNAseq profiling and LC-qTOF-MS-based metabolomics of 580 lines coupled to pathogen sensitivity assays resulted in the identification of genetic loci significantly associated with levels of hundreds of transcripts and metabolites. These associations occurred in functionally coherent hotspots that represent coordinated perturbation of metabolic pathways and ripening-related processes. The approach successfully highlighted new enzymes of the steroidal glycoalkaloid biosynthetic pathway, which we validated here experimentally. Finally, we showed that fruit resistance to Botrytis cinerea is closely associated with specific changes in the secondary metabolism and the fruit ripening regulatory network.

Scientific Publication
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