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The rice immune receptor XA21 recognizes a tyrosine-sulfated protein from a Gram-negative bacterium
Year:
2015
Source of publication :
ScienceAdvance
Authors :
Bahar, Ofir
;
.
Volume :
6
Co-Authors:
  1. Rory N. Pruitt, Benjamin Schwessinger, Anna Joe, Furong Liu, David De Vleesschauwer, Daniel Caddell,Weiguo Zhang,Xiuxiang Zhao, Xiang Li,Joshua L. Heazlewood, Deling Ruan
  2. Markus Albert, Michelle R. Robinson, Leanne Jade G. Chan, Dee Dee Luu,Huamin Chen, Arsalan Daudi, Dipali Majumder, Mawsheng Chern,Hubert Kalbacher,
  3. Samriti Midha, Prabhu B. Patil, Ramesh V. Sonti, Christopher J. Petzold, Chang C. Liu, Jennifer S. Brodbelt,Georg Felix4 and Pamela C. Ronald, 
  4.  
Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:

Surveillance of the extracellular environment by immune receptors is of central importance to eukaryotic survival. The rice receptor kinase XA21, which confers robust resistance to most strains of the Gram-negative bacterium Xanthomonas oryzae pv. oryzae (Xoo), is representative of a large class of cell surface immune receptors in plants and animals. We report the identification of a previously undescribed Xoo protein, called RaxX, which is required for activation of XA21-mediated immunity. Xoo strains that lack RaxX, or carry mutations in the single RaxX tyrosine residue (Y41), are able to evade XA21-mediated immunity. Y41 of RaxX is sulfated by the prokaryotic tyrosine sulfotransferase RaxST. Sulfated, but not nonsulfated, RaxX triggers hallmarks of the plant immune response in an XA21-dependent manner. A sulfated, 21–amino acid synthetic RaxX peptide (RaxX21-sY) is sufficient for this activity. Xoo field isolates that overcome XA21-mediated immunity encode an alternate raxX allele, suggesting that coevolutionary interactions between host and pathogen contribute to RaxX diversification. RaxX is highly conserved in many plant pathogenic Xanthomonas species. The new insights gained from the discovery and characterization of the sulfated protein, RaxX, can be applied to the development of resistant crop varieties and therapeutic reagents that have the potential to block microbial infection of both plants and animals.

Note:
Related Files :
Gram-Negative Bacteria
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More details
DOI :
10.1126/sciadv.1500245
Article number:
0
Affiliations:
Database:
Google Scholar
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
46195
Last updated date:
02/03/2022 17:27
Creation date:
17/02/2020 14:04
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Scientific Publication
The rice immune receptor XA21 recognizes a tyrosine-sulfated protein from a Gram-negative bacterium
6
  1. Rory N. Pruitt, Benjamin Schwessinger, Anna Joe, Furong Liu, David De Vleesschauwer, Daniel Caddell,Weiguo Zhang,Xiuxiang Zhao, Xiang Li,Joshua L. Heazlewood, Deling Ruan
  2. Markus Albert, Michelle R. Robinson, Leanne Jade G. Chan, Dee Dee Luu,Huamin Chen, Arsalan Daudi, Dipali Majumder, Mawsheng Chern,Hubert Kalbacher,
  3. Samriti Midha, Prabhu B. Patil, Ramesh V. Sonti, Christopher J. Petzold, Chang C. Liu, Jennifer S. Brodbelt,Georg Felix4 and Pamela C. Ronald, 
  4.  
The rice immune receptor XA21 recognizes a tyrosine-sulfated protein from a Gram-negative bacterium .

Surveillance of the extracellular environment by immune receptors is of central importance to eukaryotic survival. The rice receptor kinase XA21, which confers robust resistance to most strains of the Gram-negative bacterium Xanthomonas oryzae pv. oryzae (Xoo), is representative of a large class of cell surface immune receptors in plants and animals. We report the identification of a previously undescribed Xoo protein, called RaxX, which is required for activation of XA21-mediated immunity. Xoo strains that lack RaxX, or carry mutations in the single RaxX tyrosine residue (Y41), are able to evade XA21-mediated immunity. Y41 of RaxX is sulfated by the prokaryotic tyrosine sulfotransferase RaxST. Sulfated, but not nonsulfated, RaxX triggers hallmarks of the plant immune response in an XA21-dependent manner. A sulfated, 21–amino acid synthetic RaxX peptide (RaxX21-sY) is sufficient for this activity. Xoo field isolates that overcome XA21-mediated immunity encode an alternate raxX allele, suggesting that coevolutionary interactions between host and pathogen contribute to RaxX diversification. RaxX is highly conserved in many plant pathogenic Xanthomonas species. The new insights gained from the discovery and characterization of the sulfated protein, RaxX, can be applied to the development of resistant crop varieties and therapeutic reagents that have the potential to block microbial infection of both plants and animals.

Scientific Publication
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