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Improved method for vegetative propagation of mature Pinus halepensis and its hybrids by cuttings
Year:
2020
Source of publication :
Israel Journal of Plant Sciences
Authors :
Abu-Abied, Mohamad
;
.
David-Schwartz, Rakefet
;
.
Farkash, Lilach
;
.
Fox, Hagar
;
.
Moshe, Yossi
;
.
Sadot, Einat
;
.
Shklar, Galina
;
.
Volume :
67
Co-Authors:

 Joseph Riov. Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Israel.
Rotem Attias. Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Israel; Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Rishon Le-Zion 7505101, Israel.
Robert Sitbon. South District, Forest Management, KKL-JNF, Israel.

Facilitators :
From page:
5
To page:
15
(
Total pages:
11
)
Abstract:

Forest trees possess high genetic diversity and high heterozygosity which allow adaptation to changing environmental conditions. There is a tendency to propagate successful and unique genotypes, which are identified at their mature stage in the forests, for future improvement programs and conservation purposes. However, vegetative propagation of mature forest trees is still a challenge in many conifers. In this study, we focused on improving the rooting of cuttings of mature and old Pinus halepensis and its hybrids. We observed that storage of cuttings before rooting at 4°C for 4 weeks and prolong immersion of cuttings in a solution containing 400 mg/l of indole-3-butyric acid, 5 mg/l of the auxin conjugate 2-(2,4-dichlorophenoxy)propanoic acid-glycine methyl ester, and 0.01% of Amistar fungicide significantly improved rooting of mature cuttings. The active ingredient in Amistar is azoxystrobin, an uncoupler of respiration, which seems to directly promote rooting. Rooted cuttings of selected clones demonstrated unique and uniform growth performance, most likely delivering the intrinsic growth parameters of the mother trees. It was also observed that trees growing under drought stress possess improved rooting ability. By using rooted cuttings, it will be possible to study the relationship between growth rate and adaptation to semi-arid climate conditions. The ability to clonal propagate mature and old P. halepensis trees not only enables vegetative propagation of elite trees for improvement programs, but also provides an opportunity to preserve unique naturally occurring old P. halepensis genotypes.

Note:
Related Files :
Auxin
clonal propagation
Conservation
Pinus halepensis
rooted cuttings
uncouplers
Show More
Related Content
More details
DOI :
10.1163/22238980-20191118
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
47108
Last updated date:
02/03/2022 17:27
Creation date:
30/03/2020 17:07
Scientific Publication
Improved method for vegetative propagation of mature Pinus halepensis and its hybrids by cuttings
67

 Joseph Riov. Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Israel.
Rotem Attias. Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Israel; Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Rishon Le-Zion 7505101, Israel.
Robert Sitbon. South District, Forest Management, KKL-JNF, Israel.

Improved method for vegetative propagation of mature Pinus halepensis and its hybrids by cuttings

Forest trees possess high genetic diversity and high heterozygosity which allow adaptation to changing environmental conditions. There is a tendency to propagate successful and unique genotypes, which are identified at their mature stage in the forests, for future improvement programs and conservation purposes. However, vegetative propagation of mature forest trees is still a challenge in many conifers. In this study, we focused on improving the rooting of cuttings of mature and old Pinus halepensis and its hybrids. We observed that storage of cuttings before rooting at 4°C for 4 weeks and prolong immersion of cuttings in a solution containing 400 mg/l of indole-3-butyric acid, 5 mg/l of the auxin conjugate 2-(2,4-dichlorophenoxy)propanoic acid-glycine methyl ester, and 0.01% of Amistar fungicide significantly improved rooting of mature cuttings. The active ingredient in Amistar is azoxystrobin, an uncoupler of respiration, which seems to directly promote rooting. Rooted cuttings of selected clones demonstrated unique and uniform growth performance, most likely delivering the intrinsic growth parameters of the mother trees. It was also observed that trees growing under drought stress possess improved rooting ability. By using rooted cuttings, it will be possible to study the relationship between growth rate and adaptation to semi-arid climate conditions. The ability to clonal propagate mature and old P. halepensis trees not only enables vegetative propagation of elite trees for improvement programs, but also provides an opportunity to preserve unique naturally occurring old P. halepensis genotypes.

Scientific Publication
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