תפריט נגישות
ניגודיות עדינהניגודיות גבוהה
מונוכרום
הדגשת קישורים
חסימת אנימציה
פונט קריא
סגור
איפוס הגדרות נגישות
להורדת מודול נגישות חינם תפריט נגישותניגודיות עדינהניגודיות גבוההמונוכרוםהדגשת קישוריםחסימת אנימציהפונט קריאסגוראיפוס הגדרות נגישותלהורדת מודול נגישות חינםRapid and sensitive methods for the detection of pathogenic microorganisms are required to monitor contamination in milk and assure public health. A colorimetric antibody-based stack pad system (biosensor) was developed to detect microbiological content of milk based on nitrocellulose membranes. This modification of the lateral flow immunoassay uses a multiple-membrane biosensor containing (from top to bottom): a sample pad, a conjugation pad (with anti-Escherichia coli antibodies conjugated to horseradish peroxidase), six Escherichia coli strain DH5α bacteria-blocking layers and an absorption pad with dry substrate. Different milk samples were tested, with and without DH5α bacteria. This multistep diagnostic assay was up to 1000-fold more sensitive than the standard laboratory ELISA. This is the first step in the development of a simple and portable device for milk-safety monitoring during all processing stages, with the aim of ensuring fresh and healthy milk for consumption.
Rapid and sensitive methods for the detection of pathogenic microorganisms are required to monitor contamination in milk and assure public health. A colorimetric antibody-based stack pad system (biosensor) was developed to detect microbiological content of milk based on nitrocellulose membranes. This modification of the lateral flow immunoassay uses a multiple-membrane biosensor containing (from top to bottom): a sample pad, a conjugation pad (with anti-Escherichia coli antibodies conjugated to horseradish peroxidase), six Escherichia coli strain DH5α bacteria-blocking layers and an absorption pad with dry substrate. Different milk samples were tested, with and without DH5α bacteria. This multistep diagnostic assay was up to 1000-fold more sensitive than the standard laboratory ELISA. This is the first step in the development of a simple and portable device for milk-safety monitoring during all processing stages, with the aim of ensuring fresh and healthy milk for consumption.
