תפריט נגישות
ניגודיות עדינהניגודיות גבוהה
מונוכרום
הדגשת קישורים
חסימת אנימציה
פונט קריא
סגור
איפוס הגדרות נגישות
להורדת מודול נגישות חינם תפריט נגישותניגודיות עדינהניגודיות גבוההמונוכרוםהדגשת קישוריםחסימת אנימציהפונט קריאסגוראיפוס הגדרות נגישותלהורדת מודול נגישות חינםThe dissociation reactions of four plant viruses: citrus tristeza virus (CTV), carnation mottle virus (CarMV), carnation yellow fleck virus (CYFV), and tobacco mosaic virus (TMV) and their respective γ-globulin alkaline phosphatase conjugates, sandwiched to antibody microplates, were examined. Treatment with 0.2 M glycine-HCl buffer pH 2.2 for 60 min caused the double antibody sandwiches of CTV and CarMV to dissociate from the antibody-coated microplates. In similar treatments CYFV was eluted less efficiently. The TMV double-sandwich remained undissociated by acidification but it could be partially dissociated under alkaline conditions (pH 12.1). The application of microplate recycling for economizing routine large-scale screening of CTV is described.
The dissociation reactions of four plant viruses: citrus tristeza virus (CTV), carnation mottle virus (CarMV), carnation yellow fleck virus (CYFV), and tobacco mosaic virus (TMV) and their respective γ-globulin alkaline phosphatase conjugates, sandwiched to antibody microplates, were examined. Treatment with 0.2 M glycine-HCl buffer pH 2.2 for 60 min caused the double antibody sandwiches of CTV and CarMV to dissociate from the antibody-coated microplates. In similar treatments CYFV was eluted less efficiently. The TMV double-sandwich remained undissociated by acidification but it could be partially dissociated under alkaline conditions (pH 12.1). The application of microplate recycling for economizing routine large-scale screening of CTV is described.
