תפריט נגישות
ניגודיות עדינהניגודיות גבוהה
מונוכרום
הדגשת קישורים
חסימת אנימציה
פונט קריא
סגור
איפוס הגדרות נגישות
להורדת מודול נגישות חינם תפריט נגישותניגודיות עדינהניגודיות גבוההמונוכרוםהדגשת קישוריםחסימת אנימציהפונט קריאסגוראיפוס הגדרות נגישותלהורדת מודול נגישות חינםSatyanarayana Tatineni - Citrus Research and Education Center, University of Florida-IFAS, Lake Alfred, FL 33850, USA and USDA-ARS and Department of Plant Pathology, University of Nebraska, Lincoln, NE 68583, USA
Mohammad R. Afunian - Citrus Research and Education Center, University of Florida-IFAS, Lake Alfred, FL 33850, USA
Siddarame Gowda - Citrus Research and Education Center, University of Florida-IFAS, Lake Alfred, FL 33850, USA
Mark E. Hilf - USDA-ARS, 2001 S. Rock Road, Fort Pierce, FL 34945, USA
William O. Dawson - Citrus Research and Education Center, University of Florida-IFAS, Lake Alfred, FL 33850, USA
The members of Capillovirus genus encode two overlapping open reading frames (ORFs): ORF1 encodes a large polyprotein containing the replication-associated proteins plus a coat protein (CP), and ORF2 encodes a movement protein (MP), located within ORF1 in a different reading frame. Organization of the CP sequence as part of the replicase ORF is unusual in capilloviruses. In this study, we examined the capillovirus genome expression strategy by characterizing viral RNAs produced by Citrus tatter leaf virus (CTLV), isolate ML, a Capillovirus. CTLV-ML produced a genome-length RNA of ∼ 6.5-kb and two 3′-terminal sgRNAs in infected tissue that contain the MP and CP coding sequences (3′-sgRNA1), and the CP coding sequence (3′-sgRNA2), respectively. Both 3′-sgRNAs initiate at a conserved octanucleotide (UUG AAAGA), and are 1826 (3′-sgRNA1) and 869 (3′-sgRNA2) nts with 119 and 15 nt leader sequences, respectively, suggesting that these two 3′-sgRNAs could serve to express the MP and CP. Additionally, accumulation of two 5′-terminal sgRNAs of 5586 (5′-sgRNA1) and 4625 (5′-sgRNA2) nts was observed, and their 3′-termini mapped to 38–44 nts upstream of the transcription start sites of 3′-sgRNAs. The presence of a separate 3′-sgRNA corresponding to the CP coding sequence and its cognate 5′-terminal sgRNA (5′-sgRNA1) suggests that CTLV-ML produces a dedicated sg mRNA for the expression of its CP.
Satyanarayana Tatineni - Citrus Research and Education Center, University of Florida-IFAS, Lake Alfred, FL 33850, USA and USDA-ARS and Department of Plant Pathology, University of Nebraska, Lincoln, NE 68583, USA
Mohammad R. Afunian - Citrus Research and Education Center, University of Florida-IFAS, Lake Alfred, FL 33850, USA
Siddarame Gowda - Citrus Research and Education Center, University of Florida-IFAS, Lake Alfred, FL 33850, USA
Mark E. Hilf - USDA-ARS, 2001 S. Rock Road, Fort Pierce, FL 34945, USA
William O. Dawson - Citrus Research and Education Center, University of Florida-IFAS, Lake Alfred, FL 33850, USA
The members of Capillovirus genus encode two overlapping open reading frames (ORFs): ORF1 encodes a large polyprotein containing the replication-associated proteins plus a coat protein (CP), and ORF2 encodes a movement protein (MP), located within ORF1 in a different reading frame. Organization of the CP sequence as part of the replicase ORF is unusual in capilloviruses. In this study, we examined the capillovirus genome expression strategy by characterizing viral RNAs produced by Citrus tatter leaf virus (CTLV), isolate ML, a Capillovirus. CTLV-ML produced a genome-length RNA of ∼ 6.5-kb and two 3′-terminal sgRNAs in infected tissue that contain the MP and CP coding sequences (3′-sgRNA1), and the CP coding sequence (3′-sgRNA2), respectively. Both 3′-sgRNAs initiate at a conserved octanucleotide (UUG AAAGA), and are 1826 (3′-sgRNA1) and 869 (3′-sgRNA2) nts with 119 and 15 nt leader sequences, respectively, suggesting that these two 3′-sgRNAs could serve to express the MP and CP. Additionally, accumulation of two 5′-terminal sgRNAs of 5586 (5′-sgRNA1) and 4625 (5′-sgRNA2) nts was observed, and their 3′-termini mapped to 38–44 nts upstream of the transcription start sites of 3′-sgRNAs. The presence of a separate 3′-sgRNA corresponding to the CP coding sequence and its cognate 5′-terminal sgRNA (5′-sgRNA1) suggests that CTLV-ML produces a dedicated sg mRNA for the expression of its CP.
