תפריט נגישות
ניגודיות עדינהניגודיות גבוהה
מונוכרום
הדגשת קישורים
חסימת אנימציה
פונט קריא
סגור
איפוס הגדרות נגישות
להורדת מודול נגישות חינם תפריט נגישותניגודיות עדינהניגודיות גבוההמונוכרוםהדגשת קישוריםחסימת אנימציהפונט קריאסגוראיפוס הגדרות נגישותלהורדת מודול נגישות חינםMor Tsamir-Rimon - Plant & Environmental Sciences department, Weizmann Institute of Science, Rehovot, Israel; The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith, Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.
Shifra Ben-Dor - Life Science Core Facilities, Weizmann Institute of Science, Rehovot, 76100, Israel.
Ester Feldmesser - Life Science Core Facilities, Weizmann Institute of Science, Rehovot, 76100, Israel.
Yaara Openheimer-Shaanan - Plant & Environmental Sciences department, Weizmann Institute of Science, Rehovot, Israel.
Rakefet David-Schwartz - Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion, 7505101, Israel.
Alon Samach - The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith, Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.
Tamir Klein - Plant & Environmental Sciences department, Weizmann Institute of Science, Rehovot, Israel.
Carbon reserve use is a major drought response in trees, enabling tree survival in conditions prohibiting photosynthesis. However, regulation of starch metabolism under drought at the whole-tree scale is still poorly understood. To this end, we combined measurements of nonstructural carbohydrates (NSC), tree physiology and gene expression. The experiment was conducted outside on olive trees in pots under 90 days of seasonal spring to summer warming. Half of the trees were also subjected to limited water conditions for 28 days. Photosynthesis decreased in dehydrating trees from 19 to 0.5 µmol m-2 s-1 during the drought period. Starch degradation and mannitol production were a major drought response, with mannitol increasing to 71% and 41% out of total NSC in shoots and roots, respectively. We identified the gene family members potentially relevant either to long-term or stress-induced carbon storage. Partitioning of expression patterns among β amylase and starch synthase family members was observed, with three β amylases possibly facilitating the rapid starch degradation under heat and drought. Our results suggest a group of stress-related, starch metabolism genes, correlated with NSC fluctuations during drought and recovery. The daily starch metabolism gene expression was different from the stress-mode starch metabolism pattern, where some genes are uniquely expressed during the stress-mode response.
Mor Tsamir-Rimon - Plant & Environmental Sciences department, Weizmann Institute of Science, Rehovot, Israel; The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith, Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.
Shifra Ben-Dor - Life Science Core Facilities, Weizmann Institute of Science, Rehovot, 76100, Israel.
Ester Feldmesser - Life Science Core Facilities, Weizmann Institute of Science, Rehovot, 76100, Israel.
Yaara Openheimer-Shaanan - Plant & Environmental Sciences department, Weizmann Institute of Science, Rehovot, Israel.
Rakefet David-Schwartz - Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion, 7505101, Israel.
Alon Samach - The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith, Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.
Tamir Klein - Plant & Environmental Sciences department, Weizmann Institute of Science, Rehovot, Israel.
Carbon reserve use is a major drought response in trees, enabling tree survival in conditions prohibiting photosynthesis. However, regulation of starch metabolism under drought at the whole-tree scale is still poorly understood. To this end, we combined measurements of nonstructural carbohydrates (NSC), tree physiology and gene expression. The experiment was conducted outside on olive trees in pots under 90 days of seasonal spring to summer warming. Half of the trees were also subjected to limited water conditions for 28 days. Photosynthesis decreased in dehydrating trees from 19 to 0.5 µmol m-2 s-1 during the drought period. Starch degradation and mannitol production were a major drought response, with mannitol increasing to 71% and 41% out of total NSC in shoots and roots, respectively. We identified the gene family members potentially relevant either to long-term or stress-induced carbon storage. Partitioning of expression patterns among β amylase and starch synthase family members was observed, with three β amylases possibly facilitating the rapid starch degradation under heat and drought. Our results suggest a group of stress-related, starch metabolism genes, correlated with NSC fluctuations during drought and recovery. The daily starch metabolism gene expression was different from the stress-mode starch metabolism pattern, where some genes are uniquely expressed during the stress-mode response.
