תפריט נגישות
ניגודיות עדינהניגודיות גבוהה
מונוכרום
הדגשת קישורים
חסימת אנימציה
פונט קריא
סגור
איפוס הגדרות נגישות
להורדת מודול נגישות חינם תפריט נגישותניגודיות עדינהניגודיות גבוההמונוכרוםהדגשת קישוריםחסימת אנימציהפונט קריאסגוראיפוס הגדרות נגישותלהורדת מודול נגישות חינםM. Mevarech - Department of Microbiology, University of Tel Aviv, Israel
A method was developed for screening of virus-related RNAs in plant extracts. The method is based on the hybridization of a polynucleotide kinase-mediated, 32P-labeled, double-stranded (ds) RNA probe with RNA or sap extracts. The cucumber mosaic virus (CMV) and associated RNA-5(CARNA-5) was used as a model system. Naturally infected plants of Nicotiana glauca were screened for the presence of dsCARNA-5; dsRNA was purified on CF-11 columns and analyzed in polyacrylamide gels. Three types of low-molecular-weight CARNA-5-like dsRNA species, with estimated molecular weights of (type I) 0.27, (type II) 0.22, and (type III) 0.19 x 106 daltons, were observed in plants of N. glauca that reacted with CMV antiserum. Types II and III were shown to contain CARNA-5 sequences by Northern blot hybridization of dsRNA patterns with 32P-labeled dsCARNA-5. This labeled probe was further used to detect CARNA-5 infection in N. glauca and N. glutinosa plants by hybridization with dot-spots of dsRNA as well as plant saps. The applicability of 32P-labeled dsRNA for diagnosing specific sequences of plant virus RNAs is discussed.
M. Mevarech - Department of Microbiology, University of Tel Aviv, Israel
A method was developed for screening of virus-related RNAs in plant extracts. The method is based on the hybridization of a polynucleotide kinase-mediated, 32P-labeled, double-stranded (ds) RNA probe with RNA or sap extracts. The cucumber mosaic virus (CMV) and associated RNA-5(CARNA-5) was used as a model system. Naturally infected plants of Nicotiana glauca were screened for the presence of dsCARNA-5; dsRNA was purified on CF-11 columns and analyzed in polyacrylamide gels. Three types of low-molecular-weight CARNA-5-like dsRNA species, with estimated molecular weights of (type I) 0.27, (type II) 0.22, and (type III) 0.19 x 106 daltons, were observed in plants of N. glauca that reacted with CMV antiserum. Types II and III were shown to contain CARNA-5 sequences by Northern blot hybridization of dsRNA patterns with 32P-labeled dsCARNA-5. This labeled probe was further used to detect CARNA-5 infection in N. glauca and N. glutinosa plants by hybridization with dot-spots of dsRNA as well as plant saps. The applicability of 32P-labeled dsRNA for diagnosing specific sequences of plant virus RNAs is discussed.
