תפריט נגישות
ניגודיות עדינהניגודיות גבוהה
מונוכרום
הדגשת קישורים
חסימת אנימציה
פונט קריא
סגור
איפוס הגדרות נגישות
להורדת מודול נגישות חינם תפריט נגישותניגודיות עדינהניגודיות גבוההמונוכרוםהדגשת קישוריםחסימת אנימציהפונט קריאסגוראיפוס הגדרות נגישותלהורדת מודול נגישות חינםFrom the text:
A commentary on
Closteroviridae
by eds R. Flores, P. Moreno, B. Falk, G. P. Martelli, and W. O. Dawson
Forty years ago, an unusual name—closterovirus—was coined for an unusual group of elongated plant viruses (Bar-Joseph and Hull, 1974). This essay reflects my personal encounter with these viruses between 1966 and 1986, a period that could be considered the beginning of the emergence of the Closteroviridae as an exciting complex virus family.
The first two viruses assigned to this group, Beet yellows virus (BYV) and Citrus tristeza virus (CTV), have significant economic importance and, therefore, attracted considerable biological and epidemiological attention long before their molecular characterization. The seminal paper by Kitajima et al. (1964) reporting the association of long thread-like particles (TLP) with tristeza-expressing plants triggered much interest on the possibility of using those particles for diagnostic purposes. In 1966, I embarked on a Ph.D. project supervised by Prof. Gad Loebenstein that aimed to purify the TLP and develop a serological assay to be eventually used for the rapid detection of CTV-infected trees in case of an emerging epidemic. Isolating the long, thin, fragile TLP from woody tissue in the absence of a bioassay for quantitative estimation of the outcome of the numerous clarification, concentration and purification steps was a difficult and frustrating task. Indeed after almost three years, my attempts were still mostly unsuccessful. In retrospect, allowing me to continue the project at that stage was both remarkably generous and far-sighted. Improvements in TLP purification, including (i) the finding that young bark of only certain citrus species is the best source of TLP, (ii) the use of a careful extraction procedure and precipitation of TLP using polyethylene glycol, and (iii) the use of different combinations of buffers for extraction and resuspension allowed us to obtain sufficiently purified TLP particles to establish their viral-like composition and biophysical nature (Bar-Joseph et al., 1972), the infectivity of which was demonstrated by Garnsey et al. (1977).
From the text:
A commentary on
Closteroviridae
by eds R. Flores, P. Moreno, B. Falk, G. P. Martelli, and W. O. Dawson
Forty years ago, an unusual name—closterovirus—was coined for an unusual group of elongated plant viruses (Bar-Joseph and Hull, 1974). This essay reflects my personal encounter with these viruses between 1966 and 1986, a period that could be considered the beginning of the emergence of the Closteroviridae as an exciting complex virus family.
The first two viruses assigned to this group, Beet yellows virus (BYV) and Citrus tristeza virus (CTV), have significant economic importance and, therefore, attracted considerable biological and epidemiological attention long before their molecular characterization. The seminal paper by Kitajima et al. (1964) reporting the association of long thread-like particles (TLP) with tristeza-expressing plants triggered much interest on the possibility of using those particles for diagnostic purposes. In 1966, I embarked on a Ph.D. project supervised by Prof. Gad Loebenstein that aimed to purify the TLP and develop a serological assay to be eventually used for the rapid detection of CTV-infected trees in case of an emerging epidemic. Isolating the long, thin, fragile TLP from woody tissue in the absence of a bioassay for quantitative estimation of the outcome of the numerous clarification, concentration and purification steps was a difficult and frustrating task. Indeed after almost three years, my attempts were still mostly unsuccessful. In retrospect, allowing me to continue the project at that stage was both remarkably generous and far-sighted. Improvements in TLP purification, including (i) the finding that young bark of only certain citrus species is the best source of TLP, (ii) the use of a careful extraction procedure and precipitation of TLP using polyethylene glycol, and (iii) the use of different combinations of buffers for extraction and resuspension allowed us to obtain sufficiently purified TLP particles to establish their viral-like composition and biophysical nature (Bar-Joseph et al., 1972), the infectivity of which was demonstrated by Garnsey et al. (1977).
