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Spiroplasmas are the causal agents of citrus little‐leaf disease
Year:
1974
Source of publication :
Annals of Applied Biology
Authors :
Bar-Joseph, Moshe
;
.
Volume :
78
Co-Authors:

Markham, P.G.; Townsend, R.; Daniels, M.J.; Plaskitt, A.; Meddins, B.M.

Facilitators :
From page:
49
To page:
57
(
Total pages:
9
)
Abstract:

A spiroplasma isolated from citrus with little‐leaf disease was grown in a cell‐free medium and injected into leafhoppers (Euscelis plebejus) Injected leafhoppers, but not those fed on infected plants, transmitted the spiroplasma to white clover (Trifolium repens cv. S100) and sweet orange (Citrus sinensis cv. Valencia). Infected clover plants were severely stunted; infected sweet orange plants showed typical symptoms of citrus little‐leaf disease. The spiroplasma was detected in clover and sweet orange plants by electron microscopy; the helical morphology of the organisms was most easily recognizable in sections 150–200 nm thick. The organism was re‐isolated in cell‐free media both from infected plants and from injected E. plebejus. The original isolate and those re‐isolated from experimentally infected clover and sweet orange appeared by morphological, cultural, biochemical and serological criteria to be identical to each other and to the R8‐A2 (type) and C‐189 strains of Spiroplasma citri. Serological tests and electrophoretic analysis of protein preparations indicated no relationship to Acholeplasma laidlawii, although this organism survived for at least 10 wk after injection into E. plebejus. Our results show that the causal agent of little‐leaf disease is related to S. citri.

Note:
Related Files :
Citrus
citrus little-leaf disease
Citrus stubborn disease
plant diseases and disorders
plant protection
Spiroplasma
Show More
Related Content
More details
DOI :
https://doi.org/10.1111/j.1744-7348.1974.tb01484.x
Article number:
0
Affiliations:
Database:
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
49896
Last updated date:
02/03/2022 17:27
Creation date:
13/09/2020 13:26
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Scientific Publication
Spiroplasmas are the causal agents of citrus little‐leaf disease
78

Markham, P.G.; Townsend, R.; Daniels, M.J.; Plaskitt, A.; Meddins, B.M.

Spiroplasmas are the causal agents of citrus little‐leaf disease

A spiroplasma isolated from citrus with little‐leaf disease was grown in a cell‐free medium and injected into leafhoppers (Euscelis plebejus) Injected leafhoppers, but not those fed on infected plants, transmitted the spiroplasma to white clover (Trifolium repens cv. S100) and sweet orange (Citrus sinensis cv. Valencia). Infected clover plants were severely stunted; infected sweet orange plants showed typical symptoms of citrus little‐leaf disease. The spiroplasma was detected in clover and sweet orange plants by electron microscopy; the helical morphology of the organisms was most easily recognizable in sections 150–200 nm thick. The organism was re‐isolated in cell‐free media both from infected plants and from injected E. plebejus. The original isolate and those re‐isolated from experimentally infected clover and sweet orange appeared by morphological, cultural, biochemical and serological criteria to be identical to each other and to the R8‐A2 (type) and C‐189 strains of Spiroplasma citri. Serological tests and electrophoretic analysis of protein preparations indicated no relationship to Acholeplasma laidlawii, although this organism survived for at least 10 wk after injection into E. plebejus. Our results show that the causal agent of little‐leaf disease is related to S. citri.

Scientific Publication
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