B.Q. Manicom - Citrus and Subtropical Fruit Research Institute, Private Bag X11208, Nelspruit 1200, Republic of South Africa
J.M. Kotze - Microbiology and Plant Pathology, University of Pretoria, Pretoria 0001, Republic of South Africa
M.M. Becker - Citrus and Subtropical Fruit Research Institute, Private Bag X11208, Nelspruit 1200, South Africa
Forty-six isolates of Fusarium from diseased carnations were examined for DNA restriction fragment length polymorphisms (RFLPs) and vegetative compatibility groups (VCGs). Both RFLPs and VCGs identified two major coincident groups. RFLPs identified three minor groups which were not assigned to VCGs. Testing of subsets of the groups showed the two major groups to be pathogenic. The two methodologies give equivalent results for the genetic separation of populations, and it is suggested that once the groundwork is laid for a host/pathogen complex, these methods can replace differential hosts for the determination of taxonomic units.
B.Q. Manicom - Citrus and Subtropical Fruit Research Institute, Private Bag X11208, Nelspruit 1200, Republic of South Africa
J.M. Kotze - Microbiology and Plant Pathology, University of Pretoria, Pretoria 0001, Republic of South Africa
M.M. Becker - Citrus and Subtropical Fruit Research Institute, Private Bag X11208, Nelspruit 1200, South Africa
Forty-six isolates of Fusarium from diseased carnations were examined for DNA restriction fragment length polymorphisms (RFLPs) and vegetative compatibility groups (VCGs). Both RFLPs and VCGs identified two major coincident groups. RFLPs identified three minor groups which were not assigned to VCGs. Testing of subsets of the groups showed the two major groups to be pathogenic. The two methodologies give equivalent results for the genetic separation of populations, and it is suggested that once the groundwork is laid for a host/pathogen complex, these methods can replace differential hosts for the determination of taxonomic units.