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Phytopathogenic Mollicutes

Quan, M.V. - Plant Protection Research Institute, Duc Thang, Bac Tu Liem, Ha Noi, Viet Nam. 
Liem, N.V. -aPlant Protection Research Institute, Duc Thang, Bac Tu Liem, Ha Noi, Viet Nam

Man, L.Q. - Plant Protection Research Institute, Duc Thang, Bac Tu Liem, Ha Noi, Viet Nam 
Huy, N.Q. - Plant Protection Research Institute, Duc Thang, Bac Tu Liem, Ha Noi, Viet Nam
 
Vi, L.X. - Plant Protection Research Institute, Duc Thang, Bac Tu Liem, Ha Noi, Viet Nam

Cuong, H.V.- Vietnam National University of Agriculture, Trau Quy, Gia Lam, Ha Noi, Viet Nam 
Nguyen, D.T. - Faculty of Agriculture, Thai Nguyen University of Agriculture and Forestry, Quyet Thang, Thai Nguyen city, Viet Nam
 
Thao, H.T.B. - Faculty of Agriculture, Thai Nguyen University of Agriculture and Forestry, Quyet Thang, Thai Nguyen city, Viet Nam

Hung, N.V. - Faculty of Agriculture, Thai Nguyen University of Agriculture and Forestry, Quyet Thang, Thai Nguyen city, Viet Nam

Huy, N.D. - Vietnam National University of Agriculture, Trau Quy, Gia Lam, Ha Noi, Viet Nam

Weintraub, P.G. - Gilat Research Center, Agricultural Research Organization, Israel 
Keswani, C. - Department of Biochemistry, Institute of Science, Banaras Hindu University, Varanasi, 221005, India

Hang, L.T. - Department of Biochemistry, Institute of Science, Banaras Hindu University, Varanasi, 221005, India

Hung, N.M. - Department of Biochemistry, Institute of Science, Banaras Hindu University, Varanasi, 221005, India

Hoat, T.X. - Plant Protection Research Institute, Duc Thang, Bac Tu Liem, Ha Noi, Viet Nam


 

Sugarcane white leaf (SCWL) disease is one of the most important diseases of sugarcane in Vietnam. The presence of phytoplasmas in phloem tubes of infected plants was confirmed by DAPI staining. A nested PCR assay using R16mF2/R16mR1 and R16F2n/R16R2 primer pairs amplified products with approximate length of 1,250 bp from the symptomatic sugarcane samples. The sequence analysis indicated that the phytoplasma strains from Khanh Hoa province shared 100% sequence identity with previously sequenced SCWL phytoplasma strains that were related to ‘Candidatus Phytoplasma oryzae’ (16SrXI). The virtual RFLP patterns of a 1,127 bp sequence obtained with the restriction enzymes BfaI, BstUI, EcoRI, HaeIII, HinfI, HpaI, RsaI and TaqI indicate that this SCWL-associated phytoplasma from Vietnam is a different strain from those in the 16SrXI described ribosomal subgroups. The two insect species, Matsumuratettix hiroglyphicus and Yamatotettix flavovittatus, identified as the vectors of SCWL in Thailand, were identified and found to be infected also in the SCWL symptomatic fields in Vietnam. The transmission experiments from SCWL-infected leafhoppers to healthy sugarcane and to insect feeding medium failed, suggesting that the infected planting material is the major transmission manner of SCWL disease in Vietnam.

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A new phytoplasma strain associated with the sugarcane white leaf disease in Vietnam
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Quan, M.V. - Plant Protection Research Institute, Duc Thang, Bac Tu Liem, Ha Noi, Viet Nam. 
Liem, N.V. -aPlant Protection Research Institute, Duc Thang, Bac Tu Liem, Ha Noi, Viet Nam

Man, L.Q. - Plant Protection Research Institute, Duc Thang, Bac Tu Liem, Ha Noi, Viet Nam 
Huy, N.Q. - Plant Protection Research Institute, Duc Thang, Bac Tu Liem, Ha Noi, Viet Nam
 
Vi, L.X. - Plant Protection Research Institute, Duc Thang, Bac Tu Liem, Ha Noi, Viet Nam

Cuong, H.V.- Vietnam National University of Agriculture, Trau Quy, Gia Lam, Ha Noi, Viet Nam 
Nguyen, D.T. - Faculty of Agriculture, Thai Nguyen University of Agriculture and Forestry, Quyet Thang, Thai Nguyen city, Viet Nam
 
Thao, H.T.B. - Faculty of Agriculture, Thai Nguyen University of Agriculture and Forestry, Quyet Thang, Thai Nguyen city, Viet Nam

Hung, N.V. - Faculty of Agriculture, Thai Nguyen University of Agriculture and Forestry, Quyet Thang, Thai Nguyen city, Viet Nam

Huy, N.D. - Vietnam National University of Agriculture, Trau Quy, Gia Lam, Ha Noi, Viet Nam

Weintraub, P.G. - Gilat Research Center, Agricultural Research Organization, Israel 
Keswani, C. - Department of Biochemistry, Institute of Science, Banaras Hindu University, Varanasi, 221005, India

Hang, L.T. - Department of Biochemistry, Institute of Science, Banaras Hindu University, Varanasi, 221005, India

Hung, N.M. - Department of Biochemistry, Institute of Science, Banaras Hindu University, Varanasi, 221005, India

Hoat, T.X. - Plant Protection Research Institute, Duc Thang, Bac Tu Liem, Ha Noi, Viet Nam


 

A new phytoplasma strain associated with the sugarcane white leaf disease in Vietnam

Sugarcane white leaf (SCWL) disease is one of the most important diseases of sugarcane in Vietnam. The presence of phytoplasmas in phloem tubes of infected plants was confirmed by DAPI staining. A nested PCR assay using R16mF2/R16mR1 and R16F2n/R16R2 primer pairs amplified products with approximate length of 1,250 bp from the symptomatic sugarcane samples. The sequence analysis indicated that the phytoplasma strains from Khanh Hoa province shared 100% sequence identity with previously sequenced SCWL phytoplasma strains that were related to ‘Candidatus Phytoplasma oryzae’ (16SrXI). The virtual RFLP patterns of a 1,127 bp sequence obtained with the restriction enzymes BfaI, BstUI, EcoRI, HaeIII, HinfI, HpaI, RsaI and TaqI indicate that this SCWL-associated phytoplasma from Vietnam is a different strain from those in the 16SrXI described ribosomal subgroups. The two insect species, Matsumuratettix hiroglyphicus and Yamatotettix flavovittatus, identified as the vectors of SCWL in Thailand, were identified and found to be infected also in the SCWL symptomatic fields in Vietnam. The transmission experiments from SCWL-infected leafhoppers to healthy sugarcane and to insect feeding medium failed, suggesting that the infected planting material is the major transmission manner of SCWL disease in Vietnam.

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