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Ma, J. - Department of Postharvest Science, Institute of Postharvest and Food Sciences, the Volcani Center, Agricultural Research Organization, Bet Dagan, 50250, Israel; Key Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China

Harpaz, D. - Department of Postharvest Science, Institute of Postharvest and Food Sciences, the Volcani Center, Agricultural Research Organization, Bet Dagan, 50250, Israel; Institute of Biochemistry, Food science and Nutrition, Faculty of Agriculture, Food and Environment, the Hebrew University of Jerusalem, Rehovot, 76100, Israel

 

Liu, Y. - Key Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China; School of Food Science and Engineering, Foshan University, Foshan, 528231, China

 

Eltzov, E. - Department of Postharvest Science, Institute of Postharvest and Food Sciences, the Volcani Center, Agricultural Research Organization, Bet Dagan, 50250, Israel; Agro-Nanotechnology Research Center, Agriculture Research Organization, the Volcani Center, Rishon LeZion, 7505101, Israel

Bioluminescent bacteria whole-cell biosensors (WCBs) have been widely used in a range of sensing applications in environmental monitoring and medical diagnostics. However, most of them use planktonic bacteria cells that require complicated signal measurement processes and therefore limit the portability of the biosensor device. In this study, a simple and low-cost immobilization method was examined. The bioluminescent bioreporter bacteria was absorbed on a filter membrane disk. Further optimization of the immobilization process was conducted by comparing different surface materials (polyester and parafilm) or by adding glucose and ampicillin. The filter membrane disks with immobilized bacteria cells were stored at −20 C for three weeks without a compromise in the stability of its biosensing functionality for water toxicants monitoring. Also, the bacterial immobilized disks were integrated with smartphones-based signal detection. Then, they were exposed to water samples with ethanol, chloroform, and H2 O2, as common toxicants. The sensitivity of the smartphone-based WCB for the detection of ethanol, chloroform, and H2 O2 was 1% (v/v), 0.02% (v/v), and 0.0006% (v/v), respectively. To conclude, this bacterial immobilization approach demonstrated higher sensitivity, portability, and improved storability than the planktonic counterpart. The developed smartphone-based WCB establishes a model for future applications in the detection of environmental water toxicants. 

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Smartphone-based whole-cell biosensor platform utilizing an immobilization approach on a filter membrane disk for the monitoring of water toxicants
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Ma, J. - Department of Postharvest Science, Institute of Postharvest and Food Sciences, the Volcani Center, Agricultural Research Organization, Bet Dagan, 50250, Israel; Key Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China

Harpaz, D. - Department of Postharvest Science, Institute of Postharvest and Food Sciences, the Volcani Center, Agricultural Research Organization, Bet Dagan, 50250, Israel; Institute of Biochemistry, Food science and Nutrition, Faculty of Agriculture, Food and Environment, the Hebrew University of Jerusalem, Rehovot, 76100, Israel

 

Liu, Y. - Key Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China; School of Food Science and Engineering, Foshan University, Foshan, 528231, China

 

Eltzov, E. - Department of Postharvest Science, Institute of Postharvest and Food Sciences, the Volcani Center, Agricultural Research Organization, Bet Dagan, 50250, Israel; Agro-Nanotechnology Research Center, Agriculture Research Organization, the Volcani Center, Rishon LeZion, 7505101, Israel

Smartphone-based whole-cell biosensor platform utilizing an immobilization approach on a filter membrane disk for the monitoring of water toxicants

Bioluminescent bacteria whole-cell biosensors (WCBs) have been widely used in a range of sensing applications in environmental monitoring and medical diagnostics. However, most of them use planktonic bacteria cells that require complicated signal measurement processes and therefore limit the portability of the biosensor device. In this study, a simple and low-cost immobilization method was examined. The bioluminescent bioreporter bacteria was absorbed on a filter membrane disk. Further optimization of the immobilization process was conducted by comparing different surface materials (polyester and parafilm) or by adding glucose and ampicillin. The filter membrane disks with immobilized bacteria cells were stored at −20 C for three weeks without a compromise in the stability of its biosensing functionality for water toxicants monitoring. Also, the bacterial immobilized disks were integrated with smartphones-based signal detection. Then, they were exposed to water samples with ethanol, chloroform, and H2 O2, as common toxicants. The sensitivity of the smartphone-based WCB for the detection of ethanol, chloroform, and H2 O2 was 1% (v/v), 0.02% (v/v), and 0.0006% (v/v), respectively. To conclude, this bacterial immobilization approach demonstrated higher sensitivity, portability, and improved storability than the planktonic counterpart. The developed smartphone-based WCB establishes a model for future applications in the detection of environmental water toxicants. 

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