תפריט נגישות
ניגודיות עדינהניגודיות גבוהה
מונוכרום
הדגשת קישורים
חסימת אנימציה
פונט קריא
סגור
איפוס הגדרות נגישות
להורדת מודול נגישות חינם תפריט נגישותניגודיות עדינהניגודיות גבוההמונוכרוםהדגשת קישוריםחסימת אנימציהפונט קריאסגוראיפוס הגדרות נגישותלהורדת מודול נגישות חינםDana Ment - Department of Plant Pathology and Weed Research, Volcani CenterInstitute of Plant Protection, Agricultural Research Organization, Rishon LeZoin, Israel.
Galina Gindin - Department of Entomology and Nematology, Volcani Center, Institute of Plant Protection, Agricultural Research Organization, Rishon Le Zion, Israel.
Michael Samish - Department of Entomology and Nematology, Volcani Center, Institute of Plant Protection, Agricultural Research Organization, Rishon Le Zion, Israel; Kimron Veterinary Institute, Rishon LeZion, Israel.
Itamar Glazer - Department of Entomology and Nematology, Volcani Center, Institute of Plant Protection, Agricultural Research Organization, Rishon Le Zion, Israel.
Earlier studies demonstrated that Metarhizium brunneum, usually a broad-host pathogen of arthropods, is unable to complete its pathogenic life cycle when inoculated on the fungus-resistant tick, Hyalomma excavatum engorged females. While the fungus penetrates the cuticle of fungus-susceptible tick, Rhipicephalus annulatus females, it is unable to penetrate the cuticle of fungus-resistant tick, and even perishes on its surface. This is probably due to high concentration of antifungal fatty acids and probably also due to a hypersensitive-like response of the tick. To understand the metabolic pathways occurring in the fungal hyphae upon encountering the cuticles, we compared the response of the fungus to cuticle from susceptible and resistant tick cuticles by 2D-gels. The intracellular proteomes of M. brunneum Mb7 exposed to cuticle of the fungus-susceptible tick, R. annulatus, and to the fungus-resistant tick, H. excavatum engorged females were compared after exposure to either cuticles. By means of liquid chromatography-mass spectrometry/mass spectrometry we identified in both proteomes common proteins involved in biological processes as well as unique proteins identified only in the proteome of fungus exposed to fungus-resistant tick cuticle. These proteins were identified in high probability as heat shock proteins, four key enzymes of the glyoxylate cycle, and proteins associated with hypoxia, and exposure to antifungal drugs. These findings are discussed within the M. brunneum-tick pathosystem in relation to tick resistance and host resistance in general.
Dana Ment - Department of Plant Pathology and Weed Research, Volcani CenterInstitute of Plant Protection, Agricultural Research Organization, Rishon LeZoin, Israel.
Galina Gindin - Department of Entomology and Nematology, Volcani Center, Institute of Plant Protection, Agricultural Research Organization, Rishon Le Zion, Israel.
Michael Samish - Department of Entomology and Nematology, Volcani Center, Institute of Plant Protection, Agricultural Research Organization, Rishon Le Zion, Israel; Kimron Veterinary Institute, Rishon LeZion, Israel.
Itamar Glazer - Department of Entomology and Nematology, Volcani Center, Institute of Plant Protection, Agricultural Research Organization, Rishon Le Zion, Israel.
Earlier studies demonstrated that Metarhizium brunneum, usually a broad-host pathogen of arthropods, is unable to complete its pathogenic life cycle when inoculated on the fungus-resistant tick, Hyalomma excavatum engorged females. While the fungus penetrates the cuticle of fungus-susceptible tick, Rhipicephalus annulatus females, it is unable to penetrate the cuticle of fungus-resistant tick, and even perishes on its surface. This is probably due to high concentration of antifungal fatty acids and probably also due to a hypersensitive-like response of the tick. To understand the metabolic pathways occurring in the fungal hyphae upon encountering the cuticles, we compared the response of the fungus to cuticle from susceptible and resistant tick cuticles by 2D-gels. The intracellular proteomes of M. brunneum Mb7 exposed to cuticle of the fungus-susceptible tick, R. annulatus, and to the fungus-resistant tick, H. excavatum engorged females were compared after exposure to either cuticles. By means of liquid chromatography-mass spectrometry/mass spectrometry we identified in both proteomes common proteins involved in biological processes as well as unique proteins identified only in the proteome of fungus exposed to fungus-resistant tick cuticle. These proteins were identified in high probability as heat shock proteins, four key enzymes of the glyoxylate cycle, and proteins associated with hypoxia, and exposure to antifungal drugs. These findings are discussed within the M. brunneum-tick pathosystem in relation to tick resistance and host resistance in general.
