נגישות
menu      
Advanced Search
Syntax
Search...
Volcani treasures
About
Terms of use
Manage
Community:
אסיף מאגר המחקר החקלאי
Powered by ClearMash Solutions Ltd -
Comparative response of Metarhizium brunneum to the cuticles of susceptible and resistant hosts
Year:
2020
Authors :
Gindin, Galina
;
.
Glazer, Itamar
;
.
Ment, Dana
;
.
Samish, Michael
;
.
Volume :
Co-Authors:

Dana Ment - Department of Plant Pathology and Weed Research, Volcani CenterInstitute of Plant Protection, Agricultural Research Organization, Rishon LeZoin, Israel. 
Galina Gindin - Department of Entomology and Nematology, Volcani Center, Institute of Plant Protection, Agricultural Research Organization, Rishon Le Zion, Israel.
 
Michael Samish - Department of Entomology and Nematology, Volcani Center, Institute of Plant Protection, Agricultural Research Organization, Rishon Le Zion, Israel; Kimron Veterinary Institute, Rishon LeZion, Israel.

Itamar Glazer - Department of Entomology and Nematology, Volcani Center, Institute of Plant Protection, Agricultural Research Organization, Rishon Le Zion, Israel.

Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:

Earlier studies demonstrated that Metarhizium brunneum, usually a broad-host pathogen of arthropods, is unable to complete its pathogenic life cycle when inoculated on the fungus-resistant tick, Hyalomma excavatum engorged females. While the fungus penetrates the cuticle of fungus-susceptible tick, Rhipicephalus annulatus females, it is unable to penetrate the cuticle of fungus-resistant tick, and even perishes on its surface. This is probably due to high concentration of antifungal fatty acids and probably also due to a hypersensitive-like response of the tick. To understand the metabolic pathways occurring in the fungal hyphae upon encountering the cuticles, we compared the response of the fungus to cuticle from susceptible and resistant tick cuticles by 2D-gels. The intracellular proteomes of M. brunneum Mb7 exposed to cuticle of the fungus-susceptible tick, R. annulatus, and to the fungus-resistant tick, H. excavatum engorged females were compared after exposure to either cuticles. By means of liquid chromatography-mass spectrometry/mass spectrometry we identified in both proteomes common proteins involved in biological processes as well as unique proteins identified only in the proteome of fungus exposed to fungus-resistant tick cuticle. These proteins were identified in high probability as heat shock proteins, four key enzymes of the glyoxylate cycle, and proteins associated with hypoxia, and exposure to antifungal drugs. These findings are discussed within the M. brunneum-tick pathosystem in relation to tick resistance and host resistance in general.

Note:
Related Files :
cuticle
Entomopathogenic fungus
Hyalomma excavatum
Proteomic
Rhipicephalus annulatus
Susceptible host
tick
Show More
Related Content
More details
DOI :
10.1002/arch.21756
Article number:
0
Affiliations:
Database:
PubMed
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
51610
Last updated date:
02/03/2022 17:27
Creation date:
10/11/2020 15:45
You may also be interested in
Scientific Publication
Comparative response of Metarhizium brunneum to the cuticles of susceptible and resistant hosts

Dana Ment - Department of Plant Pathology and Weed Research, Volcani CenterInstitute of Plant Protection, Agricultural Research Organization, Rishon LeZoin, Israel. 
Galina Gindin - Department of Entomology and Nematology, Volcani Center, Institute of Plant Protection, Agricultural Research Organization, Rishon Le Zion, Israel.
 
Michael Samish - Department of Entomology and Nematology, Volcani Center, Institute of Plant Protection, Agricultural Research Organization, Rishon Le Zion, Israel; Kimron Veterinary Institute, Rishon LeZion, Israel.

Itamar Glazer - Department of Entomology and Nematology, Volcani Center, Institute of Plant Protection, Agricultural Research Organization, Rishon Le Zion, Israel.

Comparative response of Metarhizium brunneum to the cuticles of susceptible and resistant hosts

Earlier studies demonstrated that Metarhizium brunneum, usually a broad-host pathogen of arthropods, is unable to complete its pathogenic life cycle when inoculated on the fungus-resistant tick, Hyalomma excavatum engorged females. While the fungus penetrates the cuticle of fungus-susceptible tick, Rhipicephalus annulatus females, it is unable to penetrate the cuticle of fungus-resistant tick, and even perishes on its surface. This is probably due to high concentration of antifungal fatty acids and probably also due to a hypersensitive-like response of the tick. To understand the metabolic pathways occurring in the fungal hyphae upon encountering the cuticles, we compared the response of the fungus to cuticle from susceptible and resistant tick cuticles by 2D-gels. The intracellular proteomes of M. brunneum Mb7 exposed to cuticle of the fungus-susceptible tick, R. annulatus, and to the fungus-resistant tick, H. excavatum engorged females were compared after exposure to either cuticles. By means of liquid chromatography-mass spectrometry/mass spectrometry we identified in both proteomes common proteins involved in biological processes as well as unique proteins identified only in the proteome of fungus exposed to fungus-resistant tick cuticle. These proteins were identified in high probability as heat shock proteins, four key enzymes of the glyoxylate cycle, and proteins associated with hypoxia, and exposure to antifungal drugs. These findings are discussed within the M. brunneum-tick pathosystem in relation to tick resistance and host resistance in general.

Scientific Publication
You may also be interested in