תפריט נגישות
ניגודיות עדינהניגודיות גבוהה
מונוכרום
הדגשת קישורים
חסימת אנימציה
פונט קריא
סגור
איפוס הגדרות נגישות
להורדת מודול נגישות חינם תפריט נגישותניגודיות עדינהניגודיות גבוההמונוכרוםהדגשת קישוריםחסימת אנימציהפונט קריאסגוראיפוס הגדרות נגישותלהורדת מודול נגישות חינםPaula Teper-Bamnolker _Department of Postharvest and Food Sciences, The Volcani Center, ARO , Rishon LeZion, Israel.
Raz Danieli _ Department of Postharvest and Food Sciences, The Volcani Center, ARO , Rishon LeZion, Israel; Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem , Rehovot 76100, Israel.
Hadas Peled-Zehavi _ Department of Plant and Environmental Sciences, The Weizmann Institute of Science , Rehovot 76100, Israel.
Eduard Belausov _ Department of Ornamental Horticulture, The Volcani Center, ARO , Rishon LeZion, Israel.
Mohamad Abu-Abied _ Department of Ornamental Horticulture, The Volcani Center, ARO , Rishon LeZion, Israel.
Tamar Avin-Wittenberg _ Department of Plant and Environmental Sciences, Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Givat Ram , Jerusalem, 9190401, Israel.
Einat Sadot _ Department of Ornamental Horticulture, The Volcani Center, ARO , Rishon LeZion, Israel.
Dani Eshel _ Department of Postharvest and Food Sciences, The Volcani Center, ARO , Rishon LeZion, Israel.
The caspase-like vacuolar processing enzyme (VPE) is a key factor in programmed cell death (PCD) associated with plant stress responses. Growth medium lacking a carbon source and dark conditions caused punctate labeling of 35S::VPE1-GFP (StVPE1-GFP) in potato leaves. Under conditions of carbon starvation, VPE activity and PCD symptoms strongly increased in BY-2 cells, but to a much lesser extent in VPE-RNAi BY-2 cells. During extended exposure to carbon starvation, VPE expression and activity levels peaked, with a gradual increase in BY-2 cell death. Histological analysis of StVPE1-GFP in BY-2 cells showed that carbon starvation induces its translocation from the endoplasmic reticulum to the central vacuole through tonoplast engulfment. Exposure of BY-2 culture to the macroautophagy/autophagy inhibitor concanamycin A led to, along with an accumulation of autophagic bodies, accumulation of StVPE1-GFP in the cell vacuole. This accumulation did not occur in the presence of 3-methyladenine, an inhibitor of early-stage autophagy. BY-2 cells constitutively expressing RFP-StATG8IL, an autophagosome marker, showed colocalization with the StVPE1-GFP protein in the cytoplasm and vacuole. RNAi silencing of the core autophagy component ATG4 in BY-2 cells reduced VPE activity and cell death. These results are the first to suggest that VPE translocates to the cell vacuole through the autophagy pathway, leading to PCD.
Paula Teper-Bamnolker _Department of Postharvest and Food Sciences, The Volcani Center, ARO , Rishon LeZion, Israel.
Raz Danieli _ Department of Postharvest and Food Sciences, The Volcani Center, ARO , Rishon LeZion, Israel; Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem , Rehovot 76100, Israel.
Hadas Peled-Zehavi _ Department of Plant and Environmental Sciences, The Weizmann Institute of Science , Rehovot 76100, Israel.
Eduard Belausov _ Department of Ornamental Horticulture, The Volcani Center, ARO , Rishon LeZion, Israel.
Mohamad Abu-Abied _ Department of Ornamental Horticulture, The Volcani Center, ARO , Rishon LeZion, Israel.
Tamar Avin-Wittenberg _ Department of Plant and Environmental Sciences, Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Givat Ram , Jerusalem, 9190401, Israel.
Einat Sadot _ Department of Ornamental Horticulture, The Volcani Center, ARO , Rishon LeZion, Israel.
Dani Eshel _ Department of Postharvest and Food Sciences, The Volcani Center, ARO , Rishon LeZion, Israel.
The caspase-like vacuolar processing enzyme (VPE) is a key factor in programmed cell death (PCD) associated with plant stress responses. Growth medium lacking a carbon source and dark conditions caused punctate labeling of 35S::VPE1-GFP (StVPE1-GFP) in potato leaves. Under conditions of carbon starvation, VPE activity and PCD symptoms strongly increased in BY-2 cells, but to a much lesser extent in VPE-RNAi BY-2 cells. During extended exposure to carbon starvation, VPE expression and activity levels peaked, with a gradual increase in BY-2 cell death. Histological analysis of StVPE1-GFP in BY-2 cells showed that carbon starvation induces its translocation from the endoplasmic reticulum to the central vacuole through tonoplast engulfment. Exposure of BY-2 culture to the macroautophagy/autophagy inhibitor concanamycin A led to, along with an accumulation of autophagic bodies, accumulation of StVPE1-GFP in the cell vacuole. This accumulation did not occur in the presence of 3-methyladenine, an inhibitor of early-stage autophagy. BY-2 cells constitutively expressing RFP-StATG8IL, an autophagosome marker, showed colocalization with the StVPE1-GFP protein in the cytoplasm and vacuole. RNAi silencing of the core autophagy component ATG4 in BY-2 cells reduced VPE activity and cell death. These results are the first to suggest that VPE translocates to the cell vacuole through the autophagy pathway, leading to PCD.
