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Effect of chloroplast alignment and excitation wavelength on circularly polarized chlorophyll luminescence
Year:
2004
Source of publication :
Journal of Fluorescence
Authors :
Gussakovsky, Eugene
;
.
Shahak, Yosepha
;
.
Volume :
14
Co-Authors:

Barzda, V., Maksim, I., van Amerongen, H.

Facilitators :
From page:
207
To page:
216
(
Total pages:
10
)
Abstract:

Circularly polarized luminescence (CPL) is a powerful technique to study the macroorganization of photosynthetic light-harvesting apparatus in vivoand in vitro. It is particularly useful for monitoring environmental stress induced molecular re-organization of thylakoid membranes in green leaves. The current study focuses on two questions which are important to perform and interpret such experiments: how does CPL depend on the excitation wavelength and how on the orientation of the granal thylakoids. CPL and circular dichroism (CD) of pea chloroplasts were complementarily applied when chloroplasts were either in suspension or trapped in a polyacrylamide gel (PAAG) after alignment in a magnetic field. In contrast to the CD spectrum, the CPL signal was found to be independent of the excitation wavelength in both the Soret and the Q y absorption region for chloroplasts in both suspension and PAAG. The improved resolution of luminescence measurements revealed a relatively small negative CPL band in addition to the previously described large positive band. No effect of photoselection upon excitation on the CPL spectra was detected. The CPL intensity at 690 nm at the edge of the granal thylakoids was found to be higher than at the face of the grana suggesting the CPL anisotropy.

Note:
Related Files :
Anisotropy of CPL
Chiral macroaggregates of LHCII
Circular dichroism of chloroplasts
photosynthesis
plant physiology
Show More
Related Content
More details
DOI :
https://doi.org/10.1023/B:JOFL.0000016293.52545.56
Article number:
0
Affiliations:
Database:
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
52461
Last updated date:
02/03/2022 17:27
Creation date:
20/12/2020 11:32
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Scientific Publication
Effect of chloroplast alignment and excitation wavelength on circularly polarized chlorophyll luminescence
14

Barzda, V., Maksim, I., van Amerongen, H.

Effect of chloroplast alignment and excitation wavelength on circularly polarized chlorophyll luminescence .

Circularly polarized luminescence (CPL) is a powerful technique to study the macroorganization of photosynthetic light-harvesting apparatus in vivoand in vitro. It is particularly useful for monitoring environmental stress induced molecular re-organization of thylakoid membranes in green leaves. The current study focuses on two questions which are important to perform and interpret such experiments: how does CPL depend on the excitation wavelength and how on the orientation of the granal thylakoids. CPL and circular dichroism (CD) of pea chloroplasts were complementarily applied when chloroplasts were either in suspension or trapped in a polyacrylamide gel (PAAG) after alignment in a magnetic field. In contrast to the CD spectrum, the CPL signal was found to be independent of the excitation wavelength in both the Soret and the Q y absorption region for chloroplasts in both suspension and PAAG. The improved resolution of luminescence measurements revealed a relatively small negative CPL band in addition to the previously described large positive band. No effect of photoselection upon excitation on the CPL spectra was detected. The CPL intensity at 690 nm at the edge of the granal thylakoids was found to be higher than at the face of the grana suggesting the CPL anisotropy.

Scientific Publication
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