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BiP, HSP70, NDK and PDI in wheat endosperm: II. Effects of high temperature on protein and mRNA accumulation
Year:
1998
Source of publication :
Physiologia Plantarum
Authors :
Reuveni, Moshe
;
.
Volume :
103
Co-Authors:

Hurkamn, W.J., DuPont, F.M., Altenbach, S.B., Combs, A., Chan, R., Tanaka, C.K. and Bernardin, J.E.

Facilitators :
From page:
80
To page:
90
(
Total pages:
11
)
Abstract:

The effects of high temperature on accumulation of the 70‐kDa heat shock protein (HSP70) and nucleoside diphosphate kinase (NDK) as well as two other proteins that have roles in the biosynthesis of storage proteins were examined during grain development. An HSP70 homolog and a 17‐kDa NDK were co‐purified from wheat endosperm, their identity verified, and a cDNA for an HSP70 expressed in endosperm was isolated. Wheat plants (Triticum aestivum, cvs Butte and Vulcan) were heat shocked at 40°C or exposed to maximum daily temperatures of 37 or 40°C during early or mid‐grain fill. Antibodies and cDNA probes for BiP, HSP70, NDK and PDI were used to examine the effect of high temperatures on the accumulation of protein and mRNA in the endosperm. HSP70 mRNA levels increased substantially when plants were exposed to heat shock or to a 1‐day gradual increase to 40°C. The effects of a 5‐day heat treatment on mRNA levels were more complicated and depended on the developmental stage of the grain. A treatment that began at 7 days post‐anthesis (DPA) decreased the level of mRNA for HSP70, BiP, PDI and NDK, whereas a treatment that began at 14 DPA slightly increased mRNA levels. The same treatments increased the accumulation of HSP70 but did not affect BiP, PDI, or NDK protein levels. This is the first detailed report on the effects of heat on mRNA and protein levels for HSP70 in a developing seed storage tissue.

Note:

See also: Gene bank ACCESSION AF005993

Triticum aestivum 70 kDa heat shock protein (TaHSP70d) mRNA, complete cds

https://www.ncbi.nlm.nih.gov/nuccore/AF005993

Related Files :
BiP
endosperm
heat shock
Hsp70
Nucleoside diphosphate kinase
protein disulfide isomerase
wheat
Show More
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More details
DOI :
https://doi.org/10.1034/j.1399-3054.1998.1030110.x
Article number:
0
Affiliations:
Database:
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
52650
Last updated date:
02/03/2022 17:27
Creation date:
27/12/2020 15:09
You may also be interested in
Scientific Publication
BiP, HSP70, NDK and PDI in wheat endosperm: II. Effects of high temperature on protein and mRNA accumulation
103

Hurkamn, W.J., DuPont, F.M., Altenbach, S.B., Combs, A., Chan, R., Tanaka, C.K. and Bernardin, J.E.

BiP, HSP70, NDK and PDI in wheat endosperm: II. Effects of high temperature on protein and mRNA accumulation

The effects of high temperature on accumulation of the 70‐kDa heat shock protein (HSP70) and nucleoside diphosphate kinase (NDK) as well as two other proteins that have roles in the biosynthesis of storage proteins were examined during grain development. An HSP70 homolog and a 17‐kDa NDK were co‐purified from wheat endosperm, their identity verified, and a cDNA for an HSP70 expressed in endosperm was isolated. Wheat plants (Triticum aestivum, cvs Butte and Vulcan) were heat shocked at 40°C or exposed to maximum daily temperatures of 37 or 40°C during early or mid‐grain fill. Antibodies and cDNA probes for BiP, HSP70, NDK and PDI were used to examine the effect of high temperatures on the accumulation of protein and mRNA in the endosperm. HSP70 mRNA levels increased substantially when plants were exposed to heat shock or to a 1‐day gradual increase to 40°C. The effects of a 5‐day heat treatment on mRNA levels were more complicated and depended on the developmental stage of the grain. A treatment that began at 7 days post‐anthesis (DPA) decreased the level of mRNA for HSP70, BiP, PDI and NDK, whereas a treatment that began at 14 DPA slightly increased mRNA levels. The same treatments increased the accumulation of HSP70 but did not affect BiP, PDI, or NDK protein levels. This is the first detailed report on the effects of heat on mRNA and protein levels for HSP70 in a developing seed storage tissue.

See also: Gene bank ACCESSION AF005993

Triticum aestivum 70 kDa heat shock protein (TaHSP70d) mRNA, complete cds

https://www.ncbi.nlm.nih.gov/nuccore/AF005993

Scientific Publication
You may also be interested in