תפריט נגישות
ניגודיות עדינהניגודיות גבוהה
מונוכרום
הדגשת קישורים
חסימת אנימציה
פונט קריא
סגור
איפוס הגדרות נגישות
להורדת מודול נגישות חינם תפריט נגישותניגודיות עדינהניגודיות גבוההמונוכרוםהדגשת קישוריםחסימת אנימציהפונט קריאסגוראיפוס הגדרות נגישותלהורדת מודול נגישות חינםSeegehalli M Anil _ Institute of Plant Science, Agriculture Research Organization, Volcani Center, 7528809, Rishon LeZion, Israel.
Nurit Shalev _ Institute of Plant Science, Agriculture Research Organization, Volcani Center, 7528809, Rishon LeZion, Israel.
Ajjampura C Vinayaka _ Institute of Plant Science, Agriculture Research Organization, Volcani Center, 7528809, Rishon LeZion, Israel.
Stalin Nadarajan _ Institute of Plant Science, Agriculture Research Organization, Volcani Center, 7528809, Rishon LeZion, Israel.
Dvora Namdar _ Institute of Plant Science, Agriculture Research Organization, Volcani Center, 7528809, Rishon LeZion, Israel.
Eduard Belausov _ Institute of Plant Science, Agriculture Research Organization, Volcani Center, 7528809, Rishon LeZion, Israel.
Irit Shoval _ The Mina & Everard Goodman Faculty of Life Sciences, Bar-Ilan University, 5290002, Ramat Gan, Israel.
Karthik Ananth Mani _ Institute for Postharvest and Food Science, Agriculture Research Organization, Volcani Center, 7528809, Rishon LeZion, Israel.
Guy Mechrez _ Institute for Postharvest and Food Science, Agriculture Research Organization, Volcani Center, 7528809, Rishon LeZion, Israel.
Hinanit Koltai _ Institute of Plant Science, Agriculture Research Organization, Volcani Center, 7528809, Rishon LeZion, Israel.
Cannabis sativa is widely used for medical purposes and has anti-inflammatory activity. This study intended to examine the anti-inflammatory activity of cannabis on immune response markers associated with coronavirus disease 2019 (COVID-19) inflammation. An extract fraction from C. sativa Arbel strain (FCBD) substantially reduced (dose dependently) interleukin (IL)-6 and -8 levels in an alveolar epithelial (A549) cell line. FCBD contained cannabidiol (CBD), cannabigerol (CBG) and tetrahydrocannabivarin (THCV), and multiple terpenes. Treatments with FCBD and a FCBD formulation using phytocannabinoid standards (FCBD:std) reduced IL-6, IL-8, C-C Motif Chemokine Ligands (CCLs) 2 and 7, and angiotensin I converting enzyme 2 (ACE2) expression in the A549 cell line. Treatment with FCBD induced macrophage (differentiated KG1 cell line) polarization and phagocytosis in vitro, and increased CD36 and type II receptor for the Fc region of IgG (FcγRII) expression. FCBD treatment also substantially increased IL-6 and IL-8 expression in macrophages. FCBD:std, while maintaining anti-inflammatory activity in alveolar epithelial cells, led to reduced phagocytosis and pro-inflammatory IL secretion in macrophages in comparison to FCBD. The phytocannabinoid formulation may show superior activity versus the cannabis-derived fraction for reduction of lung inflammation, yet there is a need of caution proposing cannabis as treatment for COVID-19.
Seegehalli M Anil _ Institute of Plant Science, Agriculture Research Organization, Volcani Center, 7528809, Rishon LeZion, Israel.
Nurit Shalev _ Institute of Plant Science, Agriculture Research Organization, Volcani Center, 7528809, Rishon LeZion, Israel.
Ajjampura C Vinayaka _ Institute of Plant Science, Agriculture Research Organization, Volcani Center, 7528809, Rishon LeZion, Israel.
Stalin Nadarajan _ Institute of Plant Science, Agriculture Research Organization, Volcani Center, 7528809, Rishon LeZion, Israel.
Dvora Namdar _ Institute of Plant Science, Agriculture Research Organization, Volcani Center, 7528809, Rishon LeZion, Israel.
Eduard Belausov _ Institute of Plant Science, Agriculture Research Organization, Volcani Center, 7528809, Rishon LeZion, Israel.
Irit Shoval _ The Mina & Everard Goodman Faculty of Life Sciences, Bar-Ilan University, 5290002, Ramat Gan, Israel.
Karthik Ananth Mani _ Institute for Postharvest and Food Science, Agriculture Research Organization, Volcani Center, 7528809, Rishon LeZion, Israel.
Guy Mechrez _ Institute for Postharvest and Food Science, Agriculture Research Organization, Volcani Center, 7528809, Rishon LeZion, Israel.
Hinanit Koltai _ Institute of Plant Science, Agriculture Research Organization, Volcani Center, 7528809, Rishon LeZion, Israel.
Cannabis sativa is widely used for medical purposes and has anti-inflammatory activity. This study intended to examine the anti-inflammatory activity of cannabis on immune response markers associated with coronavirus disease 2019 (COVID-19) inflammation. An extract fraction from C. sativa Arbel strain (FCBD) substantially reduced (dose dependently) interleukin (IL)-6 and -8 levels in an alveolar epithelial (A549) cell line. FCBD contained cannabidiol (CBD), cannabigerol (CBG) and tetrahydrocannabivarin (THCV), and multiple terpenes. Treatments with FCBD and a FCBD formulation using phytocannabinoid standards (FCBD:std) reduced IL-6, IL-8, C-C Motif Chemokine Ligands (CCLs) 2 and 7, and angiotensin I converting enzyme 2 (ACE2) expression in the A549 cell line. Treatment with FCBD induced macrophage (differentiated KG1 cell line) polarization and phagocytosis in vitro, and increased CD36 and type II receptor for the Fc region of IgG (FcγRII) expression. FCBD treatment also substantially increased IL-6 and IL-8 expression in macrophages. FCBD:std, while maintaining anti-inflammatory activity in alveolar epithelial cells, led to reduced phagocytosis and pro-inflammatory IL secretion in macrophages in comparison to FCBD. The phytocannabinoid formulation may show superior activity versus the cannabis-derived fraction for reduction of lung inflammation, yet there is a need of caution proposing cannabis as treatment for COVID-19.
