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Improved Folch Method for Liver-Fat Quantification
Year:
2021
Source of publication :
Frontiers in Veterinary Science
Authors :
Dvir, Hay
;
.
Kalyesubula, Mugagga
;
.
Moallem, Uzi
;
.
Rozov, Alexander
;
.
Volume :
Co-Authors:

Ramgopal Mopuri   _ Volcani Center-Agricultural Research Organization, Institute of Animal Science, Rishon LeZion, Israel.
 Mugagga Kalyesubula  _ Volcani Center-Agricultural Research Organization, Institute of Animal Science, Rishon LeZion, Israel; Department of Animal Science, The Hebrew University of Jerusalem, Rehovot, Israel.
Alexander Rosov   _ Volcani Center-Agricultural Research Organization, Institute of Animal Science, Rishon LeZion, Israel. 
Nir Edery   _ Pathology Laboratory, Kimron Veterinary Institute, Veterinary Services, Rishon LeZion, Israel
Uzi Moallem   _ Volcani Center-Agricultural Research Organization, Institute of Animal Science, Rishon LeZion, Israel. 
Hay Dvir   _ Volcani Center-Agricultural Research Organization, Institute of Animal Science, Rishon LeZion, Israel.

 
Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:

Fatty liver represents a significant metabolic pathology of excess intrahepatic fat in domestic animals and humans. Quantification of hepatic-fat content is therefore essential for diagnosis and investigation of liver and metabolic disease. However, the reproducibility of hepatic steatosis analysis is often low due to subjective and technical factors. We hypothesized that improvement in tissue-lipids extraction efficiency would contribute to the accuracy and precision of liver-fat determination. To test it, we investigated the effect of standardized tissue sonication on liver-fat quantification by the Folch method in sheep. Liver samples from grownup lambs of lean (n = 16) and fatty (n = 15) livers, and from pregnant ewes (n = 6) who died from pregnancy toxemia (PT), were used for hepatic-fat content determination with or without tissue sonication. In the grown lambs, an average hepatic-fat content of 6.6% was determined in sonicated compared to 5.1% in non-sonicated specimens (P = 0.0002). Similarly, in ewes with PT, an average of 12.5% was determined with sonication compared to 10.8% without it (P = 0.0006), and the reproducibility was higher with sonication (CV of 3.1 vs. 6.1%, respectively). Thus, tissue sonication improved the efficiency of liver-lipids extraction and was significant to the accuracy and precision of hepatic-fat determination. Enzymatic quantification of triglycerides was moderately correlated with the results obtained gravimetrically (r = 0.632, P < 0.005). The reported data provide reliable reference values for pregnancy toxemic sheep. The significant improvement in liver-fat quantification observed with the reported revised protocol is likely applicable to most mammals and humans.

Note:
Related Files :
Fatty Liver
hepatic fat quantification
lipids extraction
Pregnancy toxemia
steatosis
Triglycerides
Show More
Related Content
More details
DOI :
10.3389/fvets.2020.594853
Article number:
0
Affiliations:
Database:
PubMed
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
53329
Last updated date:
02/03/2022 17:27
Creation date:
01/02/2021 16:13
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Scientific Publication
Improved Folch Method for Liver-Fat Quantification

Ramgopal Mopuri   _ Volcani Center-Agricultural Research Organization, Institute of Animal Science, Rishon LeZion, Israel.
 Mugagga Kalyesubula  _ Volcani Center-Agricultural Research Organization, Institute of Animal Science, Rishon LeZion, Israel; Department of Animal Science, The Hebrew University of Jerusalem, Rehovot, Israel.
Alexander Rosov   _ Volcani Center-Agricultural Research Organization, Institute of Animal Science, Rishon LeZion, Israel. 
Nir Edery   _ Pathology Laboratory, Kimron Veterinary Institute, Veterinary Services, Rishon LeZion, Israel
Uzi Moallem   _ Volcani Center-Agricultural Research Organization, Institute of Animal Science, Rishon LeZion, Israel. 
Hay Dvir   _ Volcani Center-Agricultural Research Organization, Institute of Animal Science, Rishon LeZion, Israel.

 
Improved Folch Method for Liver-Fat Quantification .

Fatty liver represents a significant metabolic pathology of excess intrahepatic fat in domestic animals and humans. Quantification of hepatic-fat content is therefore essential for diagnosis and investigation of liver and metabolic disease. However, the reproducibility of hepatic steatosis analysis is often low due to subjective and technical factors. We hypothesized that improvement in tissue-lipids extraction efficiency would contribute to the accuracy and precision of liver-fat determination. To test it, we investigated the effect of standardized tissue sonication on liver-fat quantification by the Folch method in sheep. Liver samples from grownup lambs of lean (n = 16) and fatty (n = 15) livers, and from pregnant ewes (n = 6) who died from pregnancy toxemia (PT), were used for hepatic-fat content determination with or without tissue sonication. In the grown lambs, an average hepatic-fat content of 6.6% was determined in sonicated compared to 5.1% in non-sonicated specimens (P = 0.0002). Similarly, in ewes with PT, an average of 12.5% was determined with sonication compared to 10.8% without it (P = 0.0006), and the reproducibility was higher with sonication (CV of 3.1 vs. 6.1%, respectively). Thus, tissue sonication improved the efficiency of liver-lipids extraction and was significant to the accuracy and precision of hepatic-fat determination. Enzymatic quantification of triglycerides was moderately correlated with the results obtained gravimetrically (r = 0.632, P < 0.005). The reported data provide reliable reference values for pregnancy toxemic sheep. The significant improvement in liver-fat quantification observed with the reported revised protocol is likely applicable to most mammals and humans.

Scientific Publication
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