Ephestia cautella pupae, 0–24 hr old, were exposed for 24 hr to a controlled atmosphere, either high in carbon dioxide or low in oxygen at 26°C. Tissue levels of glycogen, trehalose, glucose, α-glycerophosphate, glycerol, lactate, alanine, ATP, ADP, AMP, citrate and malate were determined in exposed pupae. Hypercarbia tended to increase the consumption of glycogen but had no clear effect on trehalose consumption. Glucose levels were similar to insect exposed to normal and to hypercarbic atmospheres but significantly higher in insects exposed to hypoxia. α-Glycerphosphate, glycerol, lactate and alanine levels were high in 0–24 hr old pupae but fell after 24 hr exposure to normal of hypoxic atmospheres. The levels of these metabolites fell less when nitrogen was replaced by carbon dioxide. Hypercarbia reduced the total amount of adenine nucleotides and the energy charge. However, 1% oxygen in nitrogen markedly affected the ATP level. Citrate was reduced in hypercarbia and in hypoxia, whereas malate was reduced by hypercarbia but increased in hypoxia.
Ephestia cautella pupae, 0–24 hr old, were exposed for 24 hr to a controlled atmosphere, either high in carbon dioxide or low in oxygen at 26°C. Tissue levels of glycogen, trehalose, glucose, α-glycerophosphate, glycerol, lactate, alanine, ATP, ADP, AMP, citrate and malate were determined in exposed pupae. Hypercarbia tended to increase the consumption of glycogen but had no clear effect on trehalose consumption. Glucose levels were similar to insect exposed to normal and to hypercarbic atmospheres but significantly higher in insects exposed to hypoxia. α-Glycerphosphate, glycerol, lactate and alanine levels were high in 0–24 hr old pupae but fell after 24 hr exposure to normal of hypoxic atmospheres. The levels of these metabolites fell less when nitrogen was replaced by carbon dioxide. Hypercarbia reduced the total amount of adenine nucleotides and the energy charge. However, 1% oxygen in nitrogen markedly affected the ATP level. Citrate was reduced in hypercarbia and in hypoxia, whereas malate was reduced by hypercarbia but increased in hypoxia.