תפריט נגישות
ניגודיות עדינהניגודיות גבוהה
מונוכרום
הדגשת קישורים
חסימת אנימציה
פונט קריא
סגור
איפוס הגדרות נגישות
להורדת מודול נגישות חינם תפריט נגישותניגודיות עדינהניגודיות גבוההמונוכרוםהדגשת קישוריםחסימת אנימציהפונט קריאסגוראיפוס הגדרות נגישותלהורדת מודול נגישות חינםBhusal, H.
Shemesh-Mayer, E.
Forer, I.
Kryukov, L.
Peters, R.
Kamenetsky-Goldstein, R.
Commercial garlic cultivars are propagated only vegetatively. Since viral infection is sheltered in all plant organs, it is transmitted from one generation to another. Bolting varieties of garlic often produce small bulbils in the inflorescence that can be used for propagation. We studied morphological and histological aspects of bulbil differentiation and virus transmission from the mother plant to the next generation. During inflorescence development, flowers differentiate first and then bulbils initiate between flower buds. Lignified vascular cells transport water and nutrients to the differentiating bulbils, together with virus particles that are translocated from the older organs. The two most dangerous potyviruses for garlic, OYDV and LYSV, were detected at the very early stages of garlic inflorescence differentiation, when the vascular system only began to differentiate, and later massive systemic viral infection of the mature bulbils was identified. In spite of common assumption that bulbils produce virus-free regenerants in tissue culture, RT-PCR analysis clearly shows the presence of both viruses in the regenerants originating from young inflorescences. It is concluded that without special means for virus eradication, in vitro propagation from garlic inflorescence or bulbils has no advantage in obtaining virus free propagation material.
Bhusal, H.
Shemesh-Mayer, E.
Forer, I.
Kryukov, L.
Peters, R.
Kamenetsky-Goldstein, R.
Commercial garlic cultivars are propagated only vegetatively. Since viral infection is sheltered in all plant organs, it is transmitted from one generation to another. Bolting varieties of garlic often produce small bulbils in the inflorescence that can be used for propagation. We studied morphological and histological aspects of bulbil differentiation and virus transmission from the mother plant to the next generation. During inflorescence development, flowers differentiate first and then bulbils initiate between flower buds. Lignified vascular cells transport water and nutrients to the differentiating bulbils, together with virus particles that are translocated from the older organs. The two most dangerous potyviruses for garlic, OYDV and LYSV, were detected at the very early stages of garlic inflorescence differentiation, when the vascular system only began to differentiate, and later massive systemic viral infection of the mature bulbils was identified. In spite of common assumption that bulbils produce virus-free regenerants in tissue culture, RT-PCR analysis clearly shows the presence of both viruses in the regenerants originating from young inflorescences. It is concluded that without special means for virus eradication, in vitro propagation from garlic inflorescence or bulbils has no advantage in obtaining virus free propagation material.
