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The Tomato brown rugose fruit virus movement protein overcomes Tm-2 2 resistance in tomato while attenuating viral transport
Year:
2021
Authors :
Hak, Hagit
;
.
Spiegelman, Ziv
;
.
Volume :
Co-Authors:

Hagit Hak 
Ziv Spiegelman

Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:

Tomato brown rugose fruit virus (ToBRFV) is a new virus of the Tobamovirus genus, causing substantial damage to tomato crops. Reports of recent ToBRFV outbreaks from around the world indicate an emerging global epidemic. ToBRFV overcomes all tobamovirus resistances in tomato, including the durable Tm-22 resistance gene, which had been effective against multiple tobamoviruses. Here, we show that the ToBRFV movement protein (MPToBRFV) enables the virus to evade Tm-22 resistance. Transient expression of MPToBRFV failed to activate the Tm-22 resistance response. Replacement of the original MP sequence of Tomato mosaic virus (ToMV) with MPToBRFV enabled this recombinant virus to infect Tm-22 resistant plants. Using hybrid protein analysis, we show that the elements required to evade Tm-22 are located between MPToBRFV amino acids 1 and 216, and not the C terminus as previously assumed. Analysis of ToBRFV systemic infection in tomato revealed that ToBRFV spreads slower compared to ToMV. Interestingly, replacement of Tobacco mosaic virus (TMV) and ToMV MPs with MPToBRFV caused an attenuation of systemic infection of both viruses. Cell-to-cell movement analysis showed that MPToBRFV moves less effectively compared to the TMV MP (MPTMV). These findings suggest that overcoming Tm-22 is associated with attenuated MP function. This may explain the high durability of Tm-22 resistance, which had remained unbroken for over 60 years.

Note:
Related Files :
Movement protein
Tm-2 2 resistance
tomato brown rugose fruit virus
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More details
DOI :
10.1094/MPMI-01-21-0023-R
Article number:
0
Affiliations:
Database:
PubMed
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
55001
Last updated date:
02/03/2022 17:27
Creation date:
18/05/2021 20:28
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Scientific Publication
The Tomato brown rugose fruit virus movement protein overcomes Tm-2 2 resistance in tomato while attenuating viral transport

Hagit Hak 
Ziv Spiegelman

The Tomato brown rugose fruit virus movement protein overcomes Tm-2 2 resistance in tomato while attenuating viral transport

Tomato brown rugose fruit virus (ToBRFV) is a new virus of the Tobamovirus genus, causing substantial damage to tomato crops. Reports of recent ToBRFV outbreaks from around the world indicate an emerging global epidemic. ToBRFV overcomes all tobamovirus resistances in tomato, including the durable Tm-22 resistance gene, which had been effective against multiple tobamoviruses. Here, we show that the ToBRFV movement protein (MPToBRFV) enables the virus to evade Tm-22 resistance. Transient expression of MPToBRFV failed to activate the Tm-22 resistance response. Replacement of the original MP sequence of Tomato mosaic virus (ToMV) with MPToBRFV enabled this recombinant virus to infect Tm-22 resistant plants. Using hybrid protein analysis, we show that the elements required to evade Tm-22 are located between MPToBRFV amino acids 1 and 216, and not the C terminus as previously assumed. Analysis of ToBRFV systemic infection in tomato revealed that ToBRFV spreads slower compared to ToMV. Interestingly, replacement of Tobacco mosaic virus (TMV) and ToMV MPs with MPToBRFV caused an attenuation of systemic infection of both viruses. Cell-to-cell movement analysis showed that MPToBRFV moves less effectively compared to the TMV MP (MPTMV). These findings suggest that overcoming Tm-22 is associated with attenuated MP function. This may explain the high durability of Tm-22 resistance, which had remained unbroken for over 60 years.

Scientific Publication
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