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Physiological state and photoperiod exposures differentially influence circadian rhythms of body temperature and prolactin and relate to changes in mammary PER1 expression in late pregnant and early lactation dairy goats
Year:
2021
Source of publication :
Small Ruminant Research
Authors :
Shamay, Avi
;
.
Volume :
200
Co-Authors:

Mugagga Kalyesubula
Theresa M. Casey
Naama Reicher
Chris Sabastian
Yossi Wein
Enav Bar Shira
Nguyen Hoang
Uduak Z. George
Avi Shamay
Karen Plaut
Sameer J. Mabjeesh

Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:

Short-day photoperiod (SDPP; 8 h light:16 h dark) during the dry period increases milk production compared with long-day photoperiod (LDPP; 16 h light:8 h dark). We hypothesized that the impact of photoperiod on lactation is mediated by alterations in the circadian system. Twelve Saanen goats were blocked at dry off into SDPP (n = 6) and LDPP (n = 6) treatments and mammary biopsies were taken in the middle of light and dark phases at 3 wk prepartum and 5 wk postpartum. Total RNA was isolated, and the expression of clock genes was analyzed by qPCR. SDPP goats produced more milk than LDPP goats (3.15 ± 0.04 vs. 2.7 ± 0.05 kg/d). In the 24 h period, LDPP goats had a greater body temperature than SDPP goats at 3 wk prepartum (39.6 ± 0.06 vs. 39.3 ± 0.1 °C) and 5 wk postpartum (40.1 ± 0.15 vs. 39.7 ± 0.1 °C). Cosinor analysis revealed that physiological state affected body temperature mesor (P< 0.001), peak (P< 0.01), amplitude (P< 0.05), and phase (P< 0.001). Plasma prolactin was 20, 10, and 17-fold higher in LDPP than in SDPP goats at 3 wk prepartum, 3 wk postpartum and 5 wk postpartum, respectively. Cosinor analysis revealed that photoperiod affected prolactin mesor (P< 0.0001), peak (P< 0.0001), trough (P< 0.001), amplitude (P< 0.01), and the peak to trough ratio (P< 0.01). Mammary expression of a core clock gene, PER1, was affected by the light-dark phase and the photoperiod treatment (P< 0.05). It also exhibited a photoperiod-physiological state interaction. Changes in the circadian rhythms with the onset of lactation and photoperiod manipulation support further studies of their role in the regulation of milk yield.

Note:
Related Files :
Circadian rhythms
Goat
lactation
Mammary gland
Photoperiod
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Related Content
More details
DOI :
10.1016/j.smallrumres.2021.106394
Article number:
106394
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
55139
Last updated date:
02/03/2022 17:27
Creation date:
25/05/2021 14:19
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Scientific Publication
Physiological state and photoperiod exposures differentially influence circadian rhythms of body temperature and prolactin and relate to changes in mammary PER1 expression in late pregnant and early lactation dairy goats
200

Mugagga Kalyesubula
Theresa M. Casey
Naama Reicher
Chris Sabastian
Yossi Wein
Enav Bar Shira
Nguyen Hoang
Uduak Z. George
Avi Shamay
Karen Plaut
Sameer J. Mabjeesh

Physiological state and photoperiod exposures differentially influence circadian rhythms of body temperature and prolactin and relate to changes in mammary PER1 expression in late pregnant and early lactation dairy goats

Short-day photoperiod (SDPP; 8 h light:16 h dark) during the dry period increases milk production compared with long-day photoperiod (LDPP; 16 h light:8 h dark). We hypothesized that the impact of photoperiod on lactation is mediated by alterations in the circadian system. Twelve Saanen goats were blocked at dry off into SDPP (n = 6) and LDPP (n = 6) treatments and mammary biopsies were taken in the middle of light and dark phases at 3 wk prepartum and 5 wk postpartum. Total RNA was isolated, and the expression of clock genes was analyzed by qPCR. SDPP goats produced more milk than LDPP goats (3.15 ± 0.04 vs. 2.7 ± 0.05 kg/d). In the 24 h period, LDPP goats had a greater body temperature than SDPP goats at 3 wk prepartum (39.6 ± 0.06 vs. 39.3 ± 0.1 °C) and 5 wk postpartum (40.1 ± 0.15 vs. 39.7 ± 0.1 °C). Cosinor analysis revealed that physiological state affected body temperature mesor (P< 0.001), peak (P< 0.01), amplitude (P< 0.05), and phase (P< 0.001). Plasma prolactin was 20, 10, and 17-fold higher in LDPP than in SDPP goats at 3 wk prepartum, 3 wk postpartum and 5 wk postpartum, respectively. Cosinor analysis revealed that photoperiod affected prolactin mesor (P< 0.0001), peak (P< 0.0001), trough (P< 0.001), amplitude (P< 0.01), and the peak to trough ratio (P< 0.01). Mammary expression of a core clock gene, PER1, was affected by the light-dark phase and the photoperiod treatment (P< 0.05). It also exhibited a photoperiod-physiological state interaction. Changes in the circadian rhythms with the onset of lactation and photoperiod manipulation support further studies of their role in the regulation of milk yield.

Scientific Publication
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