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Florigen governs shoot regeneration
Year:
2021
Source of publication :
Scientific Reports
Authors :
Doron-Faigenboim, Adi
;
.
Kutsher, Yaarit
;
.
Reuveni, Moshe
;
.
Volume :
Co-Authors:

Yaarit Kutsher 
Michal Fisler
Adi Faigenboim
Moshe Reuveni                

Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:

It is widely known that during the reproductive stage (flowering), plants do not root well. Most protocols of shoot regeneration in plants utilize juvenile tissue. Adding these two realities together encouraged us to study the role of florigen in shoot regeneration. Mature tobacco tissue that expresses the endogenous tobacco florigen mRNA regenerates poorly, while juvenile tissue that does not express the florigen regenerates shoots well. Inhibition of Nitric Oxide (NO) synthesis reduced shoot regeneration as well as promoted flowering and increased tobacco florigen level. In contrast, the addition of NO (by way of NO donor) to the tissue increased regeneration, delayed flowering, reduced tobacco florigen mRNA. Ectopic expression of florigen genes in tobacco or tomato decreased regeneration capacity significantly. Overexpression pear PcFT2 gene increased regeneration capacity. During regeneration, florigen mRNA was not changed. We conclude that florigen presence in mature tobacco leaves reduces roots and shoots regeneration and is the possible reason for the age-related decrease in regeneration capacity.

Note:
Related Files :
Florigen
Shoot regeneration
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Related Content
More details
DOI :
10.1038/s41598-021-93180-1
Article number:
0
Affiliations:
Database:
PubMed
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
55495
Last updated date:
02/03/2022 17:27
Creation date:
07/07/2021 15:28
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Scientific Publication
Florigen governs shoot regeneration

Yaarit Kutsher 
Michal Fisler
Adi Faigenboim
Moshe Reuveni                

Florigen governs shoot regeneration

It is widely known that during the reproductive stage (flowering), plants do not root well. Most protocols of shoot regeneration in plants utilize juvenile tissue. Adding these two realities together encouraged us to study the role of florigen in shoot regeneration. Mature tobacco tissue that expresses the endogenous tobacco florigen mRNA regenerates poorly, while juvenile tissue that does not express the florigen regenerates shoots well. Inhibition of Nitric Oxide (NO) synthesis reduced shoot regeneration as well as promoted flowering and increased tobacco florigen level. In contrast, the addition of NO (by way of NO donor) to the tissue increased regeneration, delayed flowering, reduced tobacco florigen mRNA. Ectopic expression of florigen genes in tobacco or tomato decreased regeneration capacity significantly. Overexpression pear PcFT2 gene increased regeneration capacity. During regeneration, florigen mRNA was not changed. We conclude that florigen presence in mature tobacco leaves reduces roots and shoots regeneration and is the possible reason for the age-related decrease in regeneration capacity.

Scientific Publication
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