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A Synchronized Increase of Stilbenes and Flavonoids in Metabolically Engineered Vitis vinifera cv. Gamay Red Cell Culture
Year:
2021
Authors :
Dynkin, Irina
;
.
Kumar, Varun
;
.
Oren-Shamir, Michal
;
.
Perl, Avi
;
.
Volume :
Co-Authors:

Ru Wang
Sangram Keshari Lenka 
Varun Kumar
Noga Sikron-Persi
Irena Dynkin 
David Weiss
Avichai Perl
Aaron Fait 
Michal Oren-Shamir                 

Facilitators :
From page:
0
To page:
0
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Total pages:
1
)
Abstract:

Stilbenes and flavonoids are two major health-promoting phenylpropanoid groups in grapes. Attempts to promote the accumulation of one group usually resulted in a decrease in the other. This study presents a unique strategy for simultaneously increasing metabolites in both groups in V. vinifera cv. Gamay Red grape cell culture, by overexpression of flavonol synthase (FLS) and increasing Phe availability. Increased Phe availability was achieved by transforming the cell culture with a second gene, the feedback-insensitive E. coli DAHP synthase (AroG*), and feeding them with Phe. A combined metabolomic and transcriptomic analysis reveals that the increase in both phenylpropanoid groups is accompanied by an induction of many of the flavonoid biosynthetic genes and no change in the expression levels of stilbene synthase. Furthermore, FLS overexpression with increased Phe availability resulted in higher anthocyanin levels, mainly those derived from delphinidin, due to the induction of F3'5'H. These insights may contribute to the development of grape berries with increased health benefits.

Note:
Related Files :
3-deoxy-d-arabino-heptulosonate 7-phosphate synthase
Metabolic Engineering
Phenylalanine
Vitis vinifera
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More details
DOI :
10.1021/acs.jafc.1c02119
Article number:
0
Affiliations:
Database:
PubMed
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
55525
Last updated date:
02/03/2022 17:27
Creation date:
11/07/2021 17:55
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Scientific Publication
A Synchronized Increase of Stilbenes and Flavonoids in Metabolically Engineered Vitis vinifera cv. Gamay Red Cell Culture

Ru Wang
Sangram Keshari Lenka 
Varun Kumar
Noga Sikron-Persi
Irena Dynkin 
David Weiss
Avichai Perl
Aaron Fait 
Michal Oren-Shamir                 

A Synchronized Increase of Stilbenes and Flavonoids in Metabolically Engineered Vitis vinifera cv. Gamay Red Cell Culture .

Stilbenes and flavonoids are two major health-promoting phenylpropanoid groups in grapes. Attempts to promote the accumulation of one group usually resulted in a decrease in the other. This study presents a unique strategy for simultaneously increasing metabolites in both groups in V. vinifera cv. Gamay Red grape cell culture, by overexpression of flavonol synthase (FLS) and increasing Phe availability. Increased Phe availability was achieved by transforming the cell culture with a second gene, the feedback-insensitive E. coli DAHP synthase (AroG*), and feeding them with Phe. A combined metabolomic and transcriptomic analysis reveals that the increase in both phenylpropanoid groups is accompanied by an induction of many of the flavonoid biosynthetic genes and no change in the expression levels of stilbene synthase. Furthermore, FLS overexpression with increased Phe availability resulted in higher anthocyanin levels, mainly those derived from delphinidin, due to the induction of F3'5'H. These insights may contribute to the development of grape berries with increased health benefits.

Scientific Publication
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