נגישות
menu      
Advanced Search
Syntax
Search...
Volcani treasures
About
Terms of use
Manage
Community:
אסיף מאגר המחקר החקלאי
Powered by ClearMash Solutions Ltd -
Cytokinin drives assembly of the phyllosphere microbiome and promotes disease resistance through structural and chemical cues
Year:
2021
Source of publication :
ISME Journal
Authors :
Bar, Maya
;
.
Gupta, Rupali K.
;
.
Jami, Elie
;
.
Kleiman, Maya
;
.
Leibman-Markus, Meirav
;
.
Sayas, Tali
;
.
Volume :
16
Co-Authors:
  • Rupali Gupta, 
  • Dorin Elkabetz, 
  • Meirav Leibman-Markus, 
  • Tali Sayas, 
  • Anat Schneider, 
  • Elie Jami, 
  • Maya Kleiman
  • Maya Bar 
Facilitators :
From page:
122
To page:
137
(
Total pages:
16
)
Abstract:

The plant hormone cytokinin (CK) is an important developmental regulator, promoting morphogenesis and delaying differentiation and senescence. From developmental processes, to growth, to stress tolerance, CKs are central in plant life. CKs are also known to mediate plant immunity and disease resistance, and several classes of microbes can also produce CKs, affecting the interaction with their plant hosts. While host species and genotype can be a driving force in shaping the plant microbiome, how plant developmental hormones such as CK can shape the microbiome is largely uninvestigated. Here, we examined the relationship between CK and the phyllosphere microbiome, finding that CK acts as a selective force in microbiome assembly, increasing richness, and promoting the presence of Firmicutes. CK-mediated immunity was found to partially depend on the microbial community, and bacilli isolated from previously described CK-rich plant genotypes, which overexpress a CK biosynthesis gene or have increased CK sensitivity, induced plant immunity, and promoted disease resistance. Using a biomimetic system, we investigated the relationship between the leaf microstructure, which is differentially patterned upon changes in CK content or signaling, and the growth of different phyllosphere microbes. We found that leaf structures derived from CK-rich plant genotypes support bacilli in the biomimetic system. CK was able to promote the growth, swarming, and biofilm formation of immunity inducing bacillus isolates in vitro. Overall, our results indicate that host genotype and hormonal profiles can act as a strong selective force in microbiome assembly, underlying differential immunity profiles, and pathogen resistance as a result.

Note:
Related Files :
microbiome
Plant Ecology
Show More
Related Content
More details
DOI :
10.1038/s41396-021-01060-3
Article number:
0
Affiliations:
Database:
PubMed
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
55640
Last updated date:
16/03/2022 13:07
Creation date:
19/07/2021 12:14
You may also be interested in
Scientific Publication
Cytokinin drives assembly of the phyllosphere microbiome and promotes disease resistance through structural and chemical cues
16
  • Rupali Gupta, 
  • Dorin Elkabetz, 
  • Meirav Leibman-Markus, 
  • Tali Sayas, 
  • Anat Schneider, 
  • Elie Jami, 
  • Maya Kleiman
  • Maya Bar 
Cytokinin drives assembly of the phyllosphere microbiome and promotes disease resistance through structural and chemical cues

The plant hormone cytokinin (CK) is an important developmental regulator, promoting morphogenesis and delaying differentiation and senescence. From developmental processes, to growth, to stress tolerance, CKs are central in plant life. CKs are also known to mediate plant immunity and disease resistance, and several classes of microbes can also produce CKs, affecting the interaction with their plant hosts. While host species and genotype can be a driving force in shaping the plant microbiome, how plant developmental hormones such as CK can shape the microbiome is largely uninvestigated. Here, we examined the relationship between CK and the phyllosphere microbiome, finding that CK acts as a selective force in microbiome assembly, increasing richness, and promoting the presence of Firmicutes. CK-mediated immunity was found to partially depend on the microbial community, and bacilli isolated from previously described CK-rich plant genotypes, which overexpress a CK biosynthesis gene or have increased CK sensitivity, induced plant immunity, and promoted disease resistance. Using a biomimetic system, we investigated the relationship between the leaf microstructure, which is differentially patterned upon changes in CK content or signaling, and the growth of different phyllosphere microbes. We found that leaf structures derived from CK-rich plant genotypes support bacilli in the biomimetic system. CK was able to promote the growth, swarming, and biofilm formation of immunity inducing bacillus isolates in vitro. Overall, our results indicate that host genotype and hormonal profiles can act as a strong selective force in microbiome assembly, underlying differential immunity profiles, and pathogen resistance as a result.

Scientific Publication
You may also be interested in