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Effect of the Broad Mite Polyphagotarsonemus latus on Cucumber Leaf Morphology and Defense Gene Expression [abstract]
Year:
2006
Source of publication :
Phytoparasitica
Authors :
Palevsky, Eric
;
.
Shomer, Ilan
;
.
Soroker, Victoria
;
.
Yaari, Mor
;
.
Volume :
34
Co-Authors:

R. Perl-Treves

Facilitators :
From page:
388
To page:
388
(
Total pages:
1
)
Abstract:

The broad mite (BM) Polyphagotarsonemus latus is a minute polyphagous mite that inhibits plant growth, and causes leaf distortion and fruit damage. Its mechanisms of injury and subsequent plant responses are still unclear. Time-dependent effects of BM infestation on leaf anatomy were evaluated by light microscopy and transmission electron microscopy (TEM). Using Northern analysis, we examined the expression of two genes involved in lignin synthesis, representing plant-induced defense mechanisms: peroxidase (PRX) and phenyl alanin ammonia lyase (PAL). Comparisons were made between plants infested with BM and two control groups: untreated and mechanically wounded, looking at the infested leaf and an upper leaf that was free of mites. Severe BM infestation led to complete loss of epidermis and failure of mesophyll differentiation. Hypertrophy and hyperplasia were also apparent, resulting in thick and very compact tissue, lacking air spaces. Cell walls appeared thick and distorted. A condensed layer of collapsed cells covered both sides of the infested leaf, instead of the epidermal layers. Safranin - fast green staining suggested that the wall material contained high levels of phenolic substances, presumably lignin and/or suberin. Northern analysis revealed that PRX and PAL were induced by mite feeding, whereas wounding did not induce these transcripts. PAL was also induced in the isolated upper leaf, suggesting a systemic plant response. How these responses affect the susceptibility of plants to BM infestations is yet to be studied. [L]

Note:
Related Files :
Broad mite
Cucumber
defense-related genes expression
gene expression
Leaf morphology
Polyphagotarsonemus latus
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More details
DOI :
Article number:
0
Affiliations:
Database:
Publication Type:
Abstract
;
.
Conference paper
;
.
Language:
English
Editors' remarks:
ID:
55706
Last updated date:
02/03/2022 17:27
Creation date:
22/07/2021 08:40
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Scientific Publication
Effect of the Broad Mite Polyphagotarsonemus latus on Cucumber Leaf Morphology and Defense Gene Expression [abstract]
34

R. Perl-Treves

Effect of the Broad Mite Polyphagotarsonemus latus on Cucumber Leaf Morphology and Defense Gene Expression .

The broad mite (BM) Polyphagotarsonemus latus is a minute polyphagous mite that inhibits plant growth, and causes leaf distortion and fruit damage. Its mechanisms of injury and subsequent plant responses are still unclear. Time-dependent effects of BM infestation on leaf anatomy were evaluated by light microscopy and transmission electron microscopy (TEM). Using Northern analysis, we examined the expression of two genes involved in lignin synthesis, representing plant-induced defense mechanisms: peroxidase (PRX) and phenyl alanin ammonia lyase (PAL). Comparisons were made between plants infested with BM and two control groups: untreated and mechanically wounded, looking at the infested leaf and an upper leaf that was free of mites. Severe BM infestation led to complete loss of epidermis and failure of mesophyll differentiation. Hypertrophy and hyperplasia were also apparent, resulting in thick and very compact tissue, lacking air spaces. Cell walls appeared thick and distorted. A condensed layer of collapsed cells covered both sides of the infested leaf, instead of the epidermal layers. Safranin - fast green staining suggested that the wall material contained high levels of phenolic substances, presumably lignin and/or suberin. Northern analysis revealed that PRX and PAL were induced by mite feeding, whereas wounding did not induce these transcripts. PAL was also induced in the isolated upper leaf, suggesting a systemic plant response. How these responses affect the susceptibility of plants to BM infestations is yet to be studied. [L]

Scientific Publication
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