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N-acetyl-β-D-glucosaminidase biomarker quantification in milk using Ag-porous Si SERS platform for mastitis severity evaluation
Year:
2021
Source of publication :
Applied Surface Science
Authors :
Nirala, Narsingh R.
;
.
Shtenberg, Giorgi
;
.
Volume :
566
Co-Authors:

Narsingh R. Nirala
Giorgi Shtenberg

Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:

Bovine mammary gland is susceptible to numerous bacterial infections resulting in inflammatory disease condition of bovine mastitis (BM) with a staggering economic impact on the dairy industry worldwide. Early BM detection is crucial for infection control within the dairy herd, which can be alleviated by antimicrobial therapy. N-acetyl-β-D-glucosaminidase (NAGase) is a prominent BM inflammatory biomarker secreted onto the blood circulation upon pathogenesis and then released into milk, capable of separating healthy quarters from subclinical and clinical BM cases. Herein, we report a sensitive, cost-effective and handy-to-use BM severity assay based on NAGase inherent content found in whole milk samples. Silver-coated porous Si (Ag-PSi) Fabry–Pérot interferometers were employed for quantifying the lysosomal activity in different milk qualities using surface-enhanced Raman spectroscopy (SERS). The optical response was proportional to the occurring pathogenesis induced by predominant bacteria found in dairy farms at different somatic cell levels. The optimized Ag-PSi SERS substrates were utilized for quantitative analysis of NAGase levels found at clinically relevant BM concentrations while presenting the detection limit of 0.27 μM min−1. The optical performances were associated with the conventional standardized approach in terms of recovery values (85–98%), thus presenting sufficient adaptability to complex matrix analysis as whole milk.

Note:
Related Files :
bovine mastitis
NAGase
Optical biosensor
Porous silicon
Somatic cell counts
surface-enhanced Raman spectroscopy (SERS)
Show More
Related Content
More details
DOI :
10.1016/j.apsusc.2021.150700
Article number:
150700
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
55874
Last updated date:
02/03/2022 17:27
Creation date:
08/08/2021 15:40
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Scientific Publication
N-acetyl-β-D-glucosaminidase biomarker quantification in milk using Ag-porous Si SERS platform for mastitis severity evaluation
566

Narsingh R. Nirala
Giorgi Shtenberg

N-acetyl-β-D-glucosaminidase biomarker quantification in milk using Ag-porous Si SERS platform for mastitis severity evaluation

Bovine mammary gland is susceptible to numerous bacterial infections resulting in inflammatory disease condition of bovine mastitis (BM) with a staggering economic impact on the dairy industry worldwide. Early BM detection is crucial for infection control within the dairy herd, which can be alleviated by antimicrobial therapy. N-acetyl-β-D-glucosaminidase (NAGase) is a prominent BM inflammatory biomarker secreted onto the blood circulation upon pathogenesis and then released into milk, capable of separating healthy quarters from subclinical and clinical BM cases. Herein, we report a sensitive, cost-effective and handy-to-use BM severity assay based on NAGase inherent content found in whole milk samples. Silver-coated porous Si (Ag-PSi) Fabry–Pérot interferometers were employed for quantifying the lysosomal activity in different milk qualities using surface-enhanced Raman spectroscopy (SERS). The optical response was proportional to the occurring pathogenesis induced by predominant bacteria found in dairy farms at different somatic cell levels. The optimized Ag-PSi SERS substrates were utilized for quantitative analysis of NAGase levels found at clinically relevant BM concentrations while presenting the detection limit of 0.27 μM min−1. The optical performances were associated with the conventional standardized approach in terms of recovery values (85–98%), thus presenting sufficient adaptability to complex matrix analysis as whole milk.

Scientific Publication
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