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Characterization of Pectobacterium brasiliense strains from potato and vegetables in Israel
Year:
2021
Source of publication :
Plant Pathology
Authors :
Chalupowicz, Laura
;
.
Dror, Orit
;
.
Erlich, Orly
;
.
Hazanovsky, Marina
;
.
Lebiush-Mordechai, Sara
;
.
Manulis-Sasson, Shulamit
;
.
Reuven, Michal
;
.
Tsror, Leah
;
.
Valinski, Lea
;
.
Volume :
Co-Authors:

Leah Tsror (Lahkim),
Valerie Hélias,
Sara Mordechai-Lebiush,
Orly Erlich,
Marina Hazanovsky,
Laura Chalupowicz,
Michal Reuven,
Orit Dror,
Lea Valinsky,
Angelique Laurent,
Shulamit Manulis-Sasson,

Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:

Pectobacterium brasiliense (Pbr) infects a wide range of crops worldwide, causing potato blackleg and soft rot and vegetable soft rots. This study aimed to characterize the genetic diversity and virulence variability among 68 Pbr strains isolated from either symptomless potato progeny tubers, diseased potato plants, ware potatoes wash water, or vegetables grown in Israel, as well as strains isolated from symptomless seed tubers grown in Europe, or diseased potato plants grown in France. The collection was typed using PCR and TaqMan real-time PCR analyses, dnaX sequence analysis, pulsed-field gel electrophoresis (PFGE), and pectolytic activity. dnaX phylogeny grouped almost all strains in a common genetic clade related to Pbr, which was distinct from the other Pectobacterium species. PFGE analysis identified two main clusters, including one major group of 47 strains with 95%–100% similarity. Maceration assays on two potato cultivars showed significant differences between strains but with no correlations with the source of the strains nor the status of the host (with/without symptoms). Molecular (dnaX sequences and PFGE profiles) and phenotypic analyses (tuber maceration tests) showed that the tested Pbr strains are not a homogeneous group. Analysis of the tested Pbr strains isolated from potato and vegetables grown in fields with a history of potato cultivation suggests that seed tubers imported from Europe may be the main source for Pbr in Israel. To the best of our knowledge, this is the first study that describes biodiversity and population structure of P. brasiliense isolated from potato and vegetables under hot climate conditions.

Note:
Related Files :
Pectobacterium brasiliense
potato
vegetables
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More details
DOI :
10.1111/ppa.13454
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
56386
Last updated date:
02/03/2022 17:27
Creation date:
30/09/2021 00:08
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Scientific Publication
Characterization of Pectobacterium brasiliense strains from potato and vegetables in Israel

Leah Tsror (Lahkim),
Valerie Hélias,
Sara Mordechai-Lebiush,
Orly Erlich,
Marina Hazanovsky,
Laura Chalupowicz,
Michal Reuven,
Orit Dror,
Lea Valinsky,
Angelique Laurent,
Shulamit Manulis-Sasson,

Characterization of Pectobacterium brasiliense strains from potato and vegetables in Israel

Pectobacterium brasiliense (Pbr) infects a wide range of crops worldwide, causing potato blackleg and soft rot and vegetable soft rots. This study aimed to characterize the genetic diversity and virulence variability among 68 Pbr strains isolated from either symptomless potato progeny tubers, diseased potato plants, ware potatoes wash water, or vegetables grown in Israel, as well as strains isolated from symptomless seed tubers grown in Europe, or diseased potato plants grown in France. The collection was typed using PCR and TaqMan real-time PCR analyses, dnaX sequence analysis, pulsed-field gel electrophoresis (PFGE), and pectolytic activity. dnaX phylogeny grouped almost all strains in a common genetic clade related to Pbr, which was distinct from the other Pectobacterium species. PFGE analysis identified two main clusters, including one major group of 47 strains with 95%–100% similarity. Maceration assays on two potato cultivars showed significant differences between strains but with no correlations with the source of the strains nor the status of the host (with/without symptoms). Molecular (dnaX sequences and PFGE profiles) and phenotypic analyses (tuber maceration tests) showed that the tested Pbr strains are not a homogeneous group. Analysis of the tested Pbr strains isolated from potato and vegetables grown in fields with a history of potato cultivation suggests that seed tubers imported from Europe may be the main source for Pbr in Israel. To the best of our knowledge, this is the first study that describes biodiversity and population structure of P. brasiliense isolated from potato and vegetables under hot climate conditions.

Scientific Publication
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