תפריט נגישות
ניגודיות עדינהניגודיות גבוהה
מונוכרום
הדגשת קישורים
חסימת אנימציה
פונט קריא
סגור
איפוס הגדרות נגישות
להורדת מודול נגישות חינם תפריט נגישותניגודיות עדינהניגודיות גבוההמונוכרוםהדגשת קישוריםחסימת אנימציהפונט קריאסגוראיפוס הגדרות נגישותלהורדת מודול נגישות חינםIzthak Icin
Idan Ashur
Abraham Ogungbile
Microcystins are a group of toxic cyclic heptapeptides produced by common cyanobacteria. The accumulation of microcystins in water reservoirs is an emerging worldwide problem. Exposure to microcystins is mainly associated with hepatotoxicity and carcinogenesis and leads to both acute and chronic damages. Microcystin-LR (MC-LR), the most common microcystin, accounts for most reported poisonings and is considered an imminent threat to human and animal health. Consequently, stringent regulation has recently been imposed by the World Health Organization (WHO), limiting the allowed MC-LR concentration in drinking water to <1 μg/L and a maximal daily intake of 0.04 μg/kg body weight. Current methods for MC-LR detection generally rely on chromatography coupled with mass spectrometry requiring bulky and expensive equipment, highly trained personnel, and labor-intensive preparation steps. Therefore, there is an urgent need for an affordable, on-site diagnostic tool providing a rapid quantitative determination of MC-LR in surface waters.
We have developed an electrochemical biosensor for MC-LR detection based on the transduction of bimolecular binding into an electrochemical signal. Specific antibodies were integrated with a biochip and measurement platform, applied in the detection of MC-LR by electrochemical impedance spectroscopy. Using the miniaturized platform, quantitative detection of MC-LR was feasible, exhibiting a dynamic range of five logarithmic concentrations and a limit of detection of 3 ng/L (3 part-per-trillion). This LOD is superior to currently employed solid-phase immunoassays. Furthermore, specific detection of MC-LR from models of cyanobacteria-contaminated water was demonstrated by the developed biosensor. Based on our findings, we anticipate that in the near future electrochemical biosensors would be an essential tool in water monitoring and environmental diagnostics.
Izthak Icin
Idan Ashur
Abraham Ogungbile
Microcystins are a group of toxic cyclic heptapeptides produced by common cyanobacteria. The accumulation of microcystins in water reservoirs is an emerging worldwide problem. Exposure to microcystins is mainly associated with hepatotoxicity and carcinogenesis and leads to both acute and chronic damages. Microcystin-LR (MC-LR), the most common microcystin, accounts for most reported poisonings and is considered an imminent threat to human and animal health. Consequently, stringent regulation has recently been imposed by the World Health Organization (WHO), limiting the allowed MC-LR concentration in drinking water to <1 μg/L and a maximal daily intake of 0.04 μg/kg body weight. Current methods for MC-LR detection generally rely on chromatography coupled with mass spectrometry requiring bulky and expensive equipment, highly trained personnel, and labor-intensive preparation steps. Therefore, there is an urgent need for an affordable, on-site diagnostic tool providing a rapid quantitative determination of MC-LR in surface waters.
We have developed an electrochemical biosensor for MC-LR detection based on the transduction of bimolecular binding into an electrochemical signal. Specific antibodies were integrated with a biochip and measurement platform, applied in the detection of MC-LR by electrochemical impedance spectroscopy. Using the miniaturized platform, quantitative detection of MC-LR was feasible, exhibiting a dynamic range of five logarithmic concentrations and a limit of detection of 3 ng/L (3 part-per-trillion). This LOD is superior to currently employed solid-phase immunoassays. Furthermore, specific detection of MC-LR from models of cyanobacteria-contaminated water was demonstrated by the developed biosensor. Based on our findings, we anticipate that in the near future electrochemical biosensors would be an essential tool in water monitoring and environmental diagnostics.
