אסיף מאגר המחקר החקלאי

Single-Conidium Encapsulation in Oil-in-Water Pickering Emulsions at High Encapsulation Yield

Year:

2021

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Co-Authors:

Liliya Kotliarevski
Karthik Ananth Mani
Reut Amar Feldbaum
Noga Yaakov
Eduard Belausov
Einat Zelinger
Dana Ment
Guy Mechrez

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Abstract:

This study presents an individual encapsulation of fungal conidia in an oil-in-water Pickering emulsion at a single-conidium encapsulation yield of 44%. The single-conidium encapsulation yield was characterized by analysis of confocal microscopy micrographs. Mineral oil-in-water emulsions stabilized by amine-functionalized titania dioxide (TiO₂-NH₂ or titania-NH₂) particles were prepared. The structure and the stability of the emulsions were investigated at different compositions by confocal microscopy and a LUMiSizer^® respectively. The most stable emulsions with a droplet size suitable for single-conidium encapsulation were further studied for their individual encapsulation capabilities. The yields of individual encapsulation in the emulsions; i.e., the number of conidia that were individually encapsulated out of the total number of conidia, were characterized by confocal microscopy assay. This rapid, easy to use approach to single-conidium encapsulation, which generates a significantly high yield with eco-friendly titania-based emulsions, only requires commonly used emulsification and agitation methods.

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