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Development of a portable colorimetric point-of-care device for the detection of Bacillus cereus in food specimens
Year:
2022
Source of publication :
Sensors and Actuators B: Chemical
Authors :
Eltzov, Evgeni
;
.
Harpaz, Dorin
;
.
Volume :
356
Co-Authors:

Manpreet Kaur
Dorin Harpaz
Evgeni Eltzov.

Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:

Bacillus cereus is recognized as a major foodborne pathogen that causes food poisoning and other types of infections. The development of a cost-effective and sensitive assay for the detection of this pathogenic microorganism is essential to mitigate the risk of contamination, particularly as a point-of-care device in resource-poor settings. In this study, a gelatin-based colorimetric assay was designed for the rapid detection of B. cereus in food. The multi-layer 3D-printed set-up incorporates a gelatin film of a specific concentration (10% (w/v)) that is then liquefied owing to the gelatinase activity of B. cereus. The liquefied sample passes through the colored layer causing the dye to migrate towards the bottom-placed absorption layer, thereby generating a positive colorimetric signal. The detection range of the assay (102-103 Colony-Forming Unit (CFU)/mL B. cereus) is comparable to the available methods of detection, which rely on the usage of expensive procedures. Hence, the developed assay represents a low-cost, simple, and portable point-of-care device to sensitively detect B. cereus in food specimens.

Note:
Related Files :
Bacillus cereus
Colorimetric assay
Food and foodborne pathogen
Gelatinase
Pathogenic microorganisms
Point-of-care device
Show More
Related Content
More details
DOI :
10.1016/j.snb.2021.131354
Article number:
131354
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
57843
Last updated date:
02/03/2022 17:27
Creation date:
08/02/2022 21:48
Scientific Publication
Development of a portable colorimetric point-of-care device for the detection of Bacillus cereus in food specimens
356

Manpreet Kaur
Dorin Harpaz
Evgeni Eltzov.

Development of a portable colorimetric point-of-care device for the detection of Bacillus cereus in food specimens

Bacillus cereus is recognized as a major foodborne pathogen that causes food poisoning and other types of infections. The development of a cost-effective and sensitive assay for the detection of this pathogenic microorganism is essential to mitigate the risk of contamination, particularly as a point-of-care device in resource-poor settings. In this study, a gelatin-based colorimetric assay was designed for the rapid detection of B. cereus in food. The multi-layer 3D-printed set-up incorporates a gelatin film of a specific concentration (10% (w/v)) that is then liquefied owing to the gelatinase activity of B. cereus. The liquefied sample passes through the colored layer causing the dye to migrate towards the bottom-placed absorption layer, thereby generating a positive colorimetric signal. The detection range of the assay (102-103 Colony-Forming Unit (CFU)/mL B. cereus) is comparable to the available methods of detection, which rely on the usage of expensive procedures. Hence, the developed assay represents a low-cost, simple, and portable point-of-care device to sensitively detect B. cereus in food specimens.

Scientific Publication
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