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Dual transcriptional analysis of Ocimum basilicum and Peronospora belbahrii in susceptible interactions
Year:
2022
Source of publication :
plant gene
Authors :
Dudai, Nativ
;
.
Gonda, Itay
;
.
Volume :
29
Co-Authors:

Eric T. Johnson
Hye-Seon Kim
Miaoying Tian
Nativ Dudai
Ofir Tal
Itay Gonda

Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:

Basil downy mildew, caused by the pathogen Peronospora belbahrii, is a major problem for sweet basil growers worldwide. The genome sequences of both Ocimum basilicum and P. belbahrii were recently completed but extensive transcriptome analysis of this pathosystem has not been completed. RNA sequencing was performed using basil leaf samples (of a susceptible cultivar) collected three and six days after inoculation with sporangia from an Illinois isolate of P. belbahrii and 11,890 differentially expressed basil genes were identified (q value <0.05). Gene enrichment analysis identified several Gene Ontology (GO) terms that were upregulated in inoculated basil leaves compared to mock control leaves which included groups of putative defensive genes (False Discovery Rate (FDR) < 0.05). In addition, there were several GO terms related to photosynthesis in the infected leaves that were downregulated in comparison to control leaves at statistically significant levels (FDR < 0.05). The pathogen's transcriptome data also identified three highly expressed transcripts encoding secreted glycoside hydrolases (GH, each transcript level was greater than 70,000 transcripts per million, TPM), which likely release sugars from the plant cell walls needed for the growth of the pathogen. Moreover, two of these GH transcripts were also highly expressed (greater than 30,000 TPM) in susceptible basil leaves infected by a Hawaiian isolate of Pbelbahrii in an independent experiment performed with a different basil cultivar. These results demonstrate a multilevel response of sweet basil to Pbelbahrii that is not effective enough to block the pathogen.

Note:
Related Files :
Compatible interaction
Obligate biotroph
Oomycetes
Peronospora belbahrii
Secreted effector
Sweet basil
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Related Content
More details
DOI :
10.1016/j.plgene.2021.100350
Article number:
100350
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
57887
Last updated date:
02/03/2022 17:27
Creation date:
13/02/2022 16:00
Scientific Publication
Dual transcriptional analysis of Ocimum basilicum and Peronospora belbahrii in susceptible interactions
29

Eric T. Johnson
Hye-Seon Kim
Miaoying Tian
Nativ Dudai
Ofir Tal
Itay Gonda

Dual transcriptional analysis of Ocimum basilicum and Peronospora belbahrii in susceptible interactions

Basil downy mildew, caused by the pathogen Peronospora belbahrii, is a major problem for sweet basil growers worldwide. The genome sequences of both Ocimum basilicum and P. belbahrii were recently completed but extensive transcriptome analysis of this pathosystem has not been completed. RNA sequencing was performed using basil leaf samples (of a susceptible cultivar) collected three and six days after inoculation with sporangia from an Illinois isolate of P. belbahrii and 11,890 differentially expressed basil genes were identified (q value <0.05). Gene enrichment analysis identified several Gene Ontology (GO) terms that were upregulated in inoculated basil leaves compared to mock control leaves which included groups of putative defensive genes (False Discovery Rate (FDR) < 0.05). In addition, there were several GO terms related to photosynthesis in the infected leaves that were downregulated in comparison to control leaves at statistically significant levels (FDR < 0.05). The pathogen's transcriptome data also identified three highly expressed transcripts encoding secreted glycoside hydrolases (GH, each transcript level was greater than 70,000 transcripts per million, TPM), which likely release sugars from the plant cell walls needed for the growth of the pathogen. Moreover, two of these GH transcripts were also highly expressed (greater than 30,000 TPM) in susceptible basil leaves infected by a Hawaiian isolate of Pbelbahrii in an independent experiment performed with a different basil cultivar. These results demonstrate a multilevel response of sweet basil to Pbelbahrii that is not effective enough to block the pathogen.

Scientific Publication
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