The effects of auxins, inhibitors of auxin synthesis, cytokinins and an antagonist to cytokinin activity, on embryogenesis and growth of both embryogenic and non-embryogenic habituated ovular callus lines of the «Shamouti» orange (Citrus sinensis), were studied.
While auxins (IAA, NAA) strongly inhibited embryogenesis, a marked stimulation of embryogenesis was observed after treatment with inhibitors of auxin synthesis, 5 hydroxy nitrobenzylbromide (HNB) and 7-aza indole (AZI). Kinetin 6-benzyladenine (BA), and N6-(Δ2-isopentyl) adenine (IPA) also reduced embryogenesis; Aza-guanine (AG) stimulated the embryogenic process.
The effects of auxins, inhibitors of auxin synthesis, cytokinins and an antagonist to cytokinin activity, on embryogenesis and growth of both embryogenic and non-embryogenic habituated ovular callus lines of the «Shamouti» orange (Citrus sinensis), were studied.
While auxins (IAA, NAA) strongly inhibited embryogenesis, a marked stimulation of embryogenesis was observed after treatment with inhibitors of auxin synthesis, 5 hydroxy nitrobenzylbromide (HNB) and 7-aza indole (AZI). Kinetin 6-benzyladenine (BA), and N6-(Δ2-isopentyl) adenine (IPA) also reduced embryogenesis; Aza-guanine (AG) stimulated the embryogenic process.