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Stimulation of embryogenesis in citrus ovular callus by ABA, ethephon, CCC and alar and its suppression by GA3
Year:
1978
Source of publication :
Journal of Plant Physiology
Authors :
Kochba, Joshuah
;
.
Neumann, Hanna
;
.
Saad, Shoshana
;
.
Spiegel-Roy, Pinchas
;
.
Volume :
89
Co-Authors:
Facilitators :
From page:
427
To page:
432
(
Total pages:
6
)
Abstract:

The effect of ABA (abscisic acid) on embryogenesis and growth was studied on ovular callus of the «Shamouti» orange (Citrus sinensis). Embryogenesis was considerably stimulated by 0.04–20.0 μM, with maximal response at 2–4 μM of ABA. A great stimulation of embryogenesis was also observed when low concentrations (0.01–1.0 mg/l) of 2-chloroethyl-phosphonic acid (ethephon) were added to the culture medium. Higher concentrations were strongly inhibitory.

GA3 (gibberellic acid) suppressed embryogenesis while two inhibitors of GA3 biosynthesis, CCC (2-chloroethyl trimethylammonium chloride) and Alar (succinic acid 2,2-methylhydrazide) significantly stimulated this process when added to the culture medium in the range of 0.01–10.0 mg/l.

Note:
Related Files :
ABA
Abscisic acid
CCC
Citrus
Citrus sinensis
Embryogenesis
Ethephon
GA3
gibberellic acid
Ovular callus
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Related Content
More details
DOI :
https://doi.org/10.1016/S0044-328X(78)80039-7
Article number:
0
Affiliations:
Database:
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
58127
Last updated date:
06/03/2022 16:15
Creation date:
06/03/2022 16:13
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Stimulation of embryogenesis in citrus ovular callus by ABA, ethephon, CCC and alar and its suppression by GA3
89
Stimulation of embryogenesis in citrus ovular callus by ABA, ethephon, CCC and alar and its suppression by GA3

The effect of ABA (abscisic acid) on embryogenesis and growth was studied on ovular callus of the «Shamouti» orange (Citrus sinensis). Embryogenesis was considerably stimulated by 0.04–20.0 μM, with maximal response at 2–4 μM of ABA. A great stimulation of embryogenesis was also observed when low concentrations (0.01–1.0 mg/l) of 2-chloroethyl-phosphonic acid (ethephon) were added to the culture medium. Higher concentrations were strongly inhibitory.

GA3 (gibberellic acid) suppressed embryogenesis while two inhibitors of GA3 biosynthesis, CCC (2-chloroethyl trimethylammonium chloride) and Alar (succinic acid 2,2-methylhydrazide) significantly stimulated this process when added to the culture medium in the range of 0.01–10.0 mg/l.

Scientific Publication
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