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Effect of Carbohydrates on Somatic Embryogenesis in Subcultured Nucellar Callus of Citrus Cultivars
Year:
1982
Source of publication :
Journal of Plant Physiology
Authors :
Kochba, Joshuah
;
.
Neumann, Hanna
;
.
Saad, Shoshana
;
.
Spiegel-Roy, Pinchas
;
.
Volume :
105
Co-Authors:
Facilitators :
From page:
359
To page:
368
(
Total pages:
10
)
Abstract:

The effect of several carbohydrates — sucrose, galactose, lactose, glucose and fructose — at 8 to 256 mM, on growth and embryogenesis of nucellar calli from five Citrus cultivars, was studied. While galactose and lactose over a broad concentration range enhanced embryogenesis in all calli studied, sucrose supported embryogenesis only at the lower levels tested (8–32 mM with various calli). Glucose and fructose were least effective in stimulating embryogenesis. Galactose and even more so lactose were also very effective in enhancing development of large embryos. All the sugars except fructose supported good callus growth over the concentration range tested. Addition of IAA, kinetin or GA3 to galactose, especially above 32 mM sugar, resulted in varying degrees of inhibition of embryogenesis. After 10 weeks in culture, compared with 5 weeks, the inhibition caused by IAA diminished significantly.

Note:
Related Files :
Citrus aurantium
Citrus limon
Citrus nobilis
Citrus sinensis
Embryogenesis
GA3
Galactose
IAA
kinetin
Sugars
Show More
Related Content
More details
DOI :
https://doi.org/10.1016/S0044-328X(82)80032-9
Article number:
0
Affiliations:
Database:
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
58164
Last updated date:
09/03/2022 10:20
Creation date:
09/03/2022 10:18
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Scientific Publication
Effect of Carbohydrates on Somatic Embryogenesis in Subcultured Nucellar Callus of Citrus Cultivars
105
Effect of Carbohydrates on Somatic Embryogenesis in Subcultured Nucellar Callus of Citrus Cultivars

The effect of several carbohydrates — sucrose, galactose, lactose, glucose and fructose — at 8 to 256 mM, on growth and embryogenesis of nucellar calli from five Citrus cultivars, was studied. While galactose and lactose over a broad concentration range enhanced embryogenesis in all calli studied, sucrose supported embryogenesis only at the lower levels tested (8–32 mM with various calli). Glucose and fructose were least effective in stimulating embryogenesis. Galactose and even more so lactose were also very effective in enhancing development of large embryos. All the sugars except fructose supported good callus growth over the concentration range tested. Addition of IAA, kinetin or GA3 to galactose, especially above 32 mM sugar, resulted in varying degrees of inhibition of embryogenesis. After 10 weeks in culture, compared with 5 weeks, the inhibition caused by IAA diminished significantly.

Scientific Publication
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