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Selection of Stable Salt-Tolerant Callus Cell Lines and Embryos in Citrus sinensis and C. aurantium
Year:
1982
Source of publication :
Journal of Plant Physiology
Authors :
Ben Hayyim, Gosal
;
.
Kochba, Joshuah
;
.
Neumann, Hanna
;
.
Saad, Shoshana
;
.
Spiegel-Roy, Pinchas
;
.
Volume :
106
Co-Authors:
Facilitators :
From page:
111
To page:
118
(
Total pages:
8
)
Abstract:

Several callus cell lines of «Shamouti» (Citrus sinensis Osbeck) and one cell line of «Sour orange» (C. aurantium) capable of growing in the presence of up to about 10 g l-1 NaCl, were obtained by repeated exposure of the cultured ovular cells to a medium containing salt.

Gamma irradiation prior to exposure to salt gave no selective advantage. Increased tolerance for salt was retained even after three consecutive transfers in medium without salt, indicating isolation of genetic variants exhibiting stability of the salt-tolerance trait.

Replacing NaCl by KCl resulted in significantly increased sensitivity to salt. However, when Cl- was replaced by SO4-2, the NaCl-tolerant cell lines grew well on either Na2SO4 or K2SO4. The salt-sensitive line can be induced to undergo embryogenesis in the presence of galactose. The salt-tolerant cell lines still preserve the capacity to regenerate embryos, though to a different degree. Some of these lines regenerate embryos in the presence of galactose, while others require the combination of salt and galactose in the medium. Embryos regenerated from salt-tolerant callus cells survived and grew better in saline medium than did embryos regenerated from the original salt sensitive callus.

Note:
Related Files :
Citrus
Citrus aurantium
Citrus sinensis
NaCl
Salt-Tolerance
salt tolerance (internal keyword)
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Related Content
More details
DOI :
https://doi.org/10.1016/S0044-328X(82)80073-1
Article number:
0
Affiliations:
Database:
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
58165
Last updated date:
09/03/2022 10:31
Creation date:
09/03/2022 10:24
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Selection of Stable Salt-Tolerant Callus Cell Lines and Embryos in Citrus sinensis and C. aurantium
106
Selection of Stable Salt-Tolerant Callus Cell Lines and Embryos in Citrus sinensis and C. aurantium

Several callus cell lines of «Shamouti» (Citrus sinensis Osbeck) and one cell line of «Sour orange» (C. aurantium) capable of growing in the presence of up to about 10 g l-1 NaCl, were obtained by repeated exposure of the cultured ovular cells to a medium containing salt.

Gamma irradiation prior to exposure to salt gave no selective advantage. Increased tolerance for salt was retained even after three consecutive transfers in medium without salt, indicating isolation of genetic variants exhibiting stability of the salt-tolerance trait.

Replacing NaCl by KCl resulted in significantly increased sensitivity to salt. However, when Cl- was replaced by SO4-2, the NaCl-tolerant cell lines grew well on either Na2SO4 or K2SO4. The salt-sensitive line can be induced to undergo embryogenesis in the presence of galactose. The salt-tolerant cell lines still preserve the capacity to regenerate embryos, though to a different degree. Some of these lines regenerate embryos in the presence of galactose, while others require the combination of salt and galactose in the medium. Embryos regenerated from salt-tolerant callus cells survived and grew better in saline medium than did embryos regenerated from the original salt sensitive callus.

Scientific Publication
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