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Selection for Tolerance to 2,4-Dichlorophenoxyacetic Acid in Ovular Callus of Orange (Citrus sinensis)
Year:
1983
Source of publication :
Journal of Plant Physiology
Authors :
Kochba, Joshuah
;
.
Saad, Shoshana
;
.
Spiegel-Roy, Pinchas
;
.
Volume :
109
Co-Authors:
Facilitators :
From page:
41
To page:
48
(
Total pages:
8
)
Abstract:

Growth of unselected, habituated nucellar callus of Shamouti orange (C. sinensis) ceased almost completely on media containing 5 sd 10-5 M 2,4-dichlorophenoxyacetic acid (2,4-D). Six culture lines, two of them originating from gamma-irradiated and four from non-irradiated callus, were selected. Three of the lines grew well up to 2 · 10-4 M 2,4-D, and two of these supported 4 · 10-4 2,4-D in the medium. Stability of tolerance was demonstrated. One line, R-1, has become 2,4-D dependent, while retaining embryogenic capacity in another line was accompanied by cytological change (from diploidy to tetraploidy). Embryos from the selected R-1 line tolerated media containing up to 5 · 10-5 M 2,4-D.

Note:
Related Files :
2,4-Dichlorophenoxyacetic Acid
artificial selection
Citrus
Citrus sinensis
Embryogenesis
hormone
Ploidy
selection
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More details
DOI :
https://doi.org/10.1016/S0044-328X(83)80171-8
Article number:
0
Affiliations:
Database:
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
58168
Last updated date:
09/03/2022 11:01
Creation date:
09/03/2022 10:58
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Scientific Publication
Selection for Tolerance to 2,4-Dichlorophenoxyacetic Acid in Ovular Callus of Orange (Citrus sinensis)
109
Selection for Tolerance to 2,4-Dichlorophenoxyacetic Acid in Ovular Callus of Orange (Citrus sinensis)

Growth of unselected, habituated nucellar callus of Shamouti orange (C. sinensis) ceased almost completely on media containing 5 sd 10-5 M 2,4-dichlorophenoxyacetic acid (2,4-D). Six culture lines, two of them originating from gamma-irradiated and four from non-irradiated callus, were selected. Three of the lines grew well up to 2 · 10-4 M 2,4-D, and two of these supported 4 · 10-4 2,4-D in the medium. Stability of tolerance was demonstrated. One line, R-1, has become 2,4-D dependent, while retaining embryogenic capacity in another line was accompanied by cytological change (from diploidy to tetraploidy). Embryos from the selected R-1 line tolerated media containing up to 5 · 10-5 M 2,4-D.

Scientific Publication
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