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Chitosan Treatment Promotes Wound Healing of Apple by Eliciting Phenylpropanoid Pathway and Enzymatic Browning of Wounds
Year:
2022
Source of publication :
Frontiers in Microbiology
Authors :
Prusky, Dov
;
.
Volume :
Co-Authors:

Sabina Ackah
Sulin Xue
Richard Osei
Francis Kweku-Amagloh
Yuanyuan Zong
Dov Prusky
Yang Bi

Facilitators :
From page:
0
To page:
0
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Total pages:
1
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Abstract:

Chitosan is an elicitor that induces resistance in fruits against postharvest diseases, but there is little knowledge about the wound healing ability of chitosan on apple fruits. Our study aimed at revealing the effect of chitosan on the phenylpropanoid pathway by determining some enzyme activities, products metabolites, polyphenol oxidase activity, color (L*, b*, a*), weight loss, and disease index during healing. Apple (cv. Fuji) fruits wounded artificially were treated with 2.5% chitosan and healed at 21–25°C, relative humidity = 81–85% for 7 days, and non-wounded fruits (coated and non-coated) were used as control. The result shows that chitosan treatment significantly decreased weight loss of wounded fruits and disease index of Penicillium expansum inoculated fruits. The activities of phenylalanine ammonia-lyase (PAL), cinnamic acid 4-hydroxylase (C4H), 4-coumaryl coenzyme A ligase (4CL), cinnamoyl-CoA reductase (CCR), and cinnamyl alcohol dehydrogenase (CAD) were elicited throughout the healing period by chitosan, which increased the biosynthesis of cinnamic acid, caffeic acid, ferulic acid, sinapic acid, p-coumaric acid, p-coumaryl alcohol, coniferyl alcohol, and sinapyl alcohol. Also, total phenol, flavonoid, and lignin contents were significantly increased at the fruits wounds. In addition, chitosan’s ability to enhance polyphenol oxidase activity stimulated enzymatic browning of wounds. Although wounding increased phenylpropanoid enzymes activities before healing, chitosan caused higher enzyme activities for a significant healing effect compared with the control. These findings imply that chitosan accelerates apple wound healing by activating the phenylpropanoid pathway and stimulating enzymatic browning of wounds.

Note:
Related Files :
apple fruit
Chitosan
Enzymatic browning
Phenylpropanoid metabolism
WOUND HEALING
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More details
DOI :
10.3389/fmicb.2022.828914
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
58352
Last updated date:
03/04/2022 17:45
Creation date:
03/04/2022 17:45
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Scientific Publication
Chitosan Treatment Promotes Wound Healing of Apple by Eliciting Phenylpropanoid Pathway and Enzymatic Browning of Wounds

Sabina Ackah
Sulin Xue
Richard Osei
Francis Kweku-Amagloh
Yuanyuan Zong
Dov Prusky
Yang Bi

Chitosan Treatment Promotes Wound Healing of Apple by Eliciting Phenylpropanoid Pathway and Enzymatic Browning of Wounds

Chitosan is an elicitor that induces resistance in fruits against postharvest diseases, but there is little knowledge about the wound healing ability of chitosan on apple fruits. Our study aimed at revealing the effect of chitosan on the phenylpropanoid pathway by determining some enzyme activities, products metabolites, polyphenol oxidase activity, color (L*, b*, a*), weight loss, and disease index during healing. Apple (cv. Fuji) fruits wounded artificially were treated with 2.5% chitosan and healed at 21–25°C, relative humidity = 81–85% for 7 days, and non-wounded fruits (coated and non-coated) were used as control. The result shows that chitosan treatment significantly decreased weight loss of wounded fruits and disease index of Penicillium expansum inoculated fruits. The activities of phenylalanine ammonia-lyase (PAL), cinnamic acid 4-hydroxylase (C4H), 4-coumaryl coenzyme A ligase (4CL), cinnamoyl-CoA reductase (CCR), and cinnamyl alcohol dehydrogenase (CAD) were elicited throughout the healing period by chitosan, which increased the biosynthesis of cinnamic acid, caffeic acid, ferulic acid, sinapic acid, p-coumaric acid, p-coumaryl alcohol, coniferyl alcohol, and sinapyl alcohol. Also, total phenol, flavonoid, and lignin contents were significantly increased at the fruits wounds. In addition, chitosan’s ability to enhance polyphenol oxidase activity stimulated enzymatic browning of wounds. Although wounding increased phenylpropanoid enzymes activities before healing, chitosan caused higher enzyme activities for a significant healing effect compared with the control. These findings imply that chitosan accelerates apple wound healing by activating the phenylpropanoid pathway and stimulating enzymatic browning of wounds.

Scientific Publication
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