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Over 1000-Fold Synergistic Boost in Viniferin Levels by Elicitation of Vitis vinifera cv. Gamay Red Cell Cultures over Accumulating Phenylalanine
Year:
2022
Authors :
Oren-Shamir, Michal
;
.
Sikron, Noga
;
.
Volume :
Co-Authors:
  • Ru Wang
    Varun Kumar
    Noga Sikron-Persi
    Irena Dynkin
    David Weiss
    Avichai Perl
    Aaron Fait
    Michal Oren-Shamir

Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:

Elicitation treatments of grape cell cultures with methyl jasmonate (MeJA), ultraviolet-C (UV-C) irradiation, and sucrose induce mild production of stilbenes and flavonoids due to limited substrate availability. However, these treatments cause a synergistic boost of stilbenes production when applied to two phenylalanine (Phe)-enriched transgenic grape cell lines, AroG* + STS and AroG* + FLS. The combined treatment of UV-C elicitation on the Phe-fed AroG* + STS line resulted in the highest content of stilbenes (37.8-fold increase, 17.39 mg/g dry weight (DW)) mainly due to resveratrol (64-fold, 3.23 mg/g DW) and viniferin (1343-fold, 13.43 mg/g DW). The synergistic increase following either UV-C or MeJA elicitation was due to the induction of stilbene-related genes, while sucrose treatment had no effect on gene expression levels and served as an additional carbon source for phenylpropanoids. The combined strategy presented may enable future usage of grape cell cultures for the production of stilbenes and in particular viniferin.

Note:
Related Files :
Methyl jasmonate
Phe feeding
stilbenes and flavonoids pathway
sucrose
UV-C irradiation
Vitis vinifera
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More details
DOI :
10.1021/acs.jafc.2c00107
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
58970
Last updated date:
11/05/2022 13:59
Creation date:
11/05/2022 13:58
You may also be interested in
Scientific Publication
Over 1000-Fold Synergistic Boost in Viniferin Levels by Elicitation of Vitis vinifera cv. Gamay Red Cell Cultures over Accumulating Phenylalanine
  • Ru Wang
    Varun Kumar
    Noga Sikron-Persi
    Irena Dynkin
    David Weiss
    Avichai Perl
    Aaron Fait
    Michal Oren-Shamir

Over 1000-Fold Synergistic Boost in Viniferin Levels by Elicitation of Vitis vinifera cv. Gamay Red Cell Cultures over Accumulating Phenylalanine

Elicitation treatments of grape cell cultures with methyl jasmonate (MeJA), ultraviolet-C (UV-C) irradiation, and sucrose induce mild production of stilbenes and flavonoids due to limited substrate availability. However, these treatments cause a synergistic boost of stilbenes production when applied to two phenylalanine (Phe)-enriched transgenic grape cell lines, AroG* + STS and AroG* + FLS. The combined treatment of UV-C elicitation on the Phe-fed AroG* + STS line resulted in the highest content of stilbenes (37.8-fold increase, 17.39 mg/g dry weight (DW)) mainly due to resveratrol (64-fold, 3.23 mg/g DW) and viniferin (1343-fold, 13.43 mg/g DW). The synergistic increase following either UV-C or MeJA elicitation was due to the induction of stilbene-related genes, while sucrose treatment had no effect on gene expression levels and served as an additional carbon source for phenylpropanoids. The combined strategy presented may enable future usage of grape cell cultures for the production of stilbenes and in particular viniferin.

Scientific Publication
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