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Molecular characterization of phospholipase c (AaPLC) in infection structures differentiation induced by pear fruit surface signals, stress responses, secondary metabolism and virulence of Alternaria alternata
Year:
2022
Source of publication :
Phytopathology
Authors :
Prusky, Dov
;
.
Volume :
Co-Authors:

Yi Huang
Yongcai Li
Dongmei Li
Yang Bi
Yongxiang Liu
Renyan Mao
Miao Zhang
Qianqian Jiang
Xiaojing Wang
Dov Prusky

Facilitators :
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Total pages:
1
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Abstract:

Fungal pathogens use plant surface physiochemical signals to trigger specific developmental processes. To assess the role of Phospholipase C (PLC) in mediating plant stimuli sensing of Alternaria alternata, function of three PLC genes were characterized by constructing ΔAaPLC mutants. Here we showed that the fruit wax-coated surfaces significantly induced appressorium formation in A. alternata and mutants (P<0.05). Germination of ΔAaPLC mutants did not differ from the wild-type. Deletion of AaPLC1 led to a reduction in appressorium and infected hyphae, but it was still recognized the stimulation from different waxes, with the strongest response to pear wax. Appressorium formation and infected hyphae of the ΔAaPLC1 mutant on dewaxed onion epidermis mounted with pear wax (θ4) were reduced by 14.5 % and 65.7% after 8 h incubation (P<0.05), while the ΔAaPLC2 and ΔAaPLC3 formed the same infection hyphae as the wild-type (P>0.05). In addition, AaPLC1 mutation caused pleiotropic effects on fungal biological function, including growth deficiency, changes in stress tolerance, weakening of pathogenicity to the host, as well as destruction of mycotoxin synthesis. Both the AaPLC2 and AaPLC3 genes were found to have some effects on stress response and mycotoxin production. Taken together, AaPLC genes differentially regulate the growth, stress response, pathogenicity and secondary metabolism of A. alternata.

Note:
Related Files :
Fungal pathogens
Pathogen Recognition by Plants
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DOI :
10.1094/PHYTO-11-21-0475-R
Article number:
0
Affiliations:
Database:
PubMed
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
59218
Last updated date:
06/06/2022 15:49
Creation date:
06/06/2022 15:48
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Scientific Publication
Molecular characterization of phospholipase c (AaPLC) in infection structures differentiation induced by pear fruit surface signals, stress responses, secondary metabolism and virulence of Alternaria alternata

Yi Huang
Yongcai Li
Dongmei Li
Yang Bi
Yongxiang Liu
Renyan Mao
Miao Zhang
Qianqian Jiang
Xiaojing Wang
Dov Prusky

Molecular characterization of phospholipase c (AaPLC) in infection structures differentiation induced by pear fruit surface signals, stress responses, secondary metabolism and virulence of Alternaria alternata

Fungal pathogens use plant surface physiochemical signals to trigger specific developmental processes. To assess the role of Phospholipase C (PLC) in mediating plant stimuli sensing of Alternaria alternata, function of three PLC genes were characterized by constructing ΔAaPLC mutants. Here we showed that the fruit wax-coated surfaces significantly induced appressorium formation in A. alternata and mutants (P<0.05). Germination of ΔAaPLC mutants did not differ from the wild-type. Deletion of AaPLC1 led to a reduction in appressorium and infected hyphae, but it was still recognized the stimulation from different waxes, with the strongest response to pear wax. Appressorium formation and infected hyphae of the ΔAaPLC1 mutant on dewaxed onion epidermis mounted with pear wax (θ4) were reduced by 14.5 % and 65.7% after 8 h incubation (P<0.05), while the ΔAaPLC2 and ΔAaPLC3 formed the same infection hyphae as the wild-type (P>0.05). In addition, AaPLC1 mutation caused pleiotropic effects on fungal biological function, including growth deficiency, changes in stress tolerance, weakening of pathogenicity to the host, as well as destruction of mycotoxin synthesis. Both the AaPLC2 and AaPLC3 genes were found to have some effects on stress response and mycotoxin production. Taken together, AaPLC genes differentially regulate the growth, stress response, pathogenicity and secondary metabolism of A. alternata.

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