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Identification and characterization of three nearly identical linalool/nerolidol synthase from Acorus calamus
Year:
2022
Source of publication :
Phytochemistry
Authors :
Ibdah, Mwafaq
;
.
Nawade, Bhagwat
;
.
Yahyaa, Mosaab
;
.
Volume :
Co-Authors:

Mwafaq Ibdah
Shada Hino
Bhagwat Nawade
Mosaab Yahyaa
Tejas C. Bosamia
Liora Shaltiel-Harpaz

Facilitators :
From page:
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Total pages:
1
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Abstract:

Acorus calamus is a perennial aromatic medicinal plant from the Acorusaceae family, known for its pharmaceutical and medicinal value. A combined chemical, biochemical, and molecular study was conducted to evaluate the differential accumulation of volatile organic compounds (VOCs) in rhizomes and leaves of A. calamus essential oil. Here, we performed VOC profiling and transcriptome-based identification and functional characterization of terpene synthase (TPS) genes. A total of 110 VOCs were detected from the rhizomes and leaves of A. calamus, and some VOCs showed significant differences between them. The further transcriptome-based analysis led to the identification of six putative TPSs genes. In phylogenetic analysis, three TPSs belonged to the TPS-g clade, one to each of the TPS-a, TPS-c, and TPS-e clades. The heterologous E. coli-based expression of recombinant TPSs identified three genes (AcTPS3, AcTPS4, and AcTPS5) as bifunctional linalool/nerolidol synthase. The correlation of TPS gene expression and VOC metabolite profiles supported the function of these genes in A. calamus. Our findings provide a roadmap for future efforts to enhance the molecular mechanisms of terpene biosynthesis and our understanding of Acorus-insect interactions.

Note:
Related Files :
Acorusaceae
Acorus calamus
Chemical compositions
terpene synthase
Volatiles
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Related Content
More details
DOI :
10.1016/j.phytochem.2022.113318
Article number:
113318
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
61485
Last updated date:
22/08/2022 13:04
Creation date:
21/08/2022 17:24
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Scientific Publication
Identification and characterization of three nearly identical linalool/nerolidol synthase from Acorus calamus

Mwafaq Ibdah
Shada Hino
Bhagwat Nawade
Mosaab Yahyaa
Tejas C. Bosamia
Liora Shaltiel-Harpaz

Identification and characterization of three nearly identical linalool/nerolidol synthase from Acorus calamus

Acorus calamus is a perennial aromatic medicinal plant from the Acorusaceae family, known for its pharmaceutical and medicinal value. A combined chemical, biochemical, and molecular study was conducted to evaluate the differential accumulation of volatile organic compounds (VOCs) in rhizomes and leaves of A. calamus essential oil. Here, we performed VOC profiling and transcriptome-based identification and functional characterization of terpene synthase (TPS) genes. A total of 110 VOCs were detected from the rhizomes and leaves of A. calamus, and some VOCs showed significant differences between them. The further transcriptome-based analysis led to the identification of six putative TPSs genes. In phylogenetic analysis, three TPSs belonged to the TPS-g clade, one to each of the TPS-a, TPS-c, and TPS-e clades. The heterologous E. coli-based expression of recombinant TPSs identified three genes (AcTPS3, AcTPS4, and AcTPS5) as bifunctional linalool/nerolidol synthase. The correlation of TPS gene expression and VOC metabolite profiles supported the function of these genes in A. calamus. Our findings provide a roadmap for future efforts to enhance the molecular mechanisms of terpene biosynthesis and our understanding of Acorus-insect interactions.

Scientific Publication
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