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Members of the BrowZine Journal Cover Fusarium oxysporum Complex Causing Wilt Symptoms in Medical Cannabis in Israel, Italy, and North America Comprise a Polyphyletic Assemblage
Year:
2022
Source of publication :
Plant Disease
Authors :
Freeman, Stanley
;
.
Jerushalmi, Shachar
;
.
Maymon, Marcel
;
.
Volume :
Co-Authors:

Shachar Jerushalmi 
Marcel Maymon
Kerry O'Donnell
Stanley Freeman 

Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:

Members of the Fusarium oxysporum complex are ubiquitous soilborne fungal pathogens causing wilt diseases in various plant hosts. Fusarium oxysporum (Fo) f. sp. cannabis was first reported causing wilt disease in hemp in Italy in 1962. To date, Fusarium wilt continues to cause concern in industrial and medicinal cannabis cultivation worldwide. During a 3-year period (2018 to 2021), Fo strains were isolated from medical cannabis plants (Cannabis sativa) exhibiting wilt symptoms that were cultivated in numerous commercial farms in Israel. A diverse set of these strains was subjected to molecular phylogenetic analyses to assess their genetic diversity and to compare them with other f. sp. cannabis isolates included in prior studies. Maximum likelihood bootstrap analysis of a partial translation elongation factor (TEF1) dataset, which included 24 f. sp. cannabis sequences, revealed that the 11 strains from Israel comprised five TEF1 haplotypes. Two of the haplotypes from Israel were identical to isolates previously reported from British Columbia and California and British Columbia and Ontario. Overall, the 24 f. sp. cannabis sequences included 12 unique TEF1 haplotypes. These were phylogenetically diverse, suggesting that pathogenicity to C. sativa may have evolved independently within the F. oxysporum complex. Pathogenicity tests of the Israeli strains were confirmed by Koch's postulates assays. Strains of the five different f. sp. cannabis TEF1 haplotypes all caused wilt in cannabis seedlings but with varying levels of aggressiveness. The same isolates that originated from asymptomatic infected mother plants were found in wilted cuttings indicating that the pathogen can be spread via propagation material.

Note:
Related Files :
Fusarium
Medical Cannabis
phylogeny
wilt
Show More
Related Content
More details
DOI :
10.1094/PDIS-01-22-0155-RE
Article number:
0
Affiliations:
Database:
PubMed
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
62334
Last updated date:
03/10/2022 18:55
Creation date:
03/10/2022 18:54
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Scientific Publication
Members of the BrowZine Journal Cover Fusarium oxysporum Complex Causing Wilt Symptoms in Medical Cannabis in Israel, Italy, and North America Comprise a Polyphyletic Assemblage

Shachar Jerushalmi 
Marcel Maymon
Kerry O'Donnell
Stanley Freeman 

Members of the BrowZine Journal Cover Fusarium oxysporum Complex Causing Wilt Symptoms in Medical Cannabis in Israel, Italy, and North America Comprise a Polyphyletic Assemblage

Members of the Fusarium oxysporum complex are ubiquitous soilborne fungal pathogens causing wilt diseases in various plant hosts. Fusarium oxysporum (Fo) f. sp. cannabis was first reported causing wilt disease in hemp in Italy in 1962. To date, Fusarium wilt continues to cause concern in industrial and medicinal cannabis cultivation worldwide. During a 3-year period (2018 to 2021), Fo strains were isolated from medical cannabis plants (Cannabis sativa) exhibiting wilt symptoms that were cultivated in numerous commercial farms in Israel. A diverse set of these strains was subjected to molecular phylogenetic analyses to assess their genetic diversity and to compare them with other f. sp. cannabis isolates included in prior studies. Maximum likelihood bootstrap analysis of a partial translation elongation factor (TEF1) dataset, which included 24 f. sp. cannabis sequences, revealed that the 11 strains from Israel comprised five TEF1 haplotypes. Two of the haplotypes from Israel were identical to isolates previously reported from British Columbia and California and British Columbia and Ontario. Overall, the 24 f. sp. cannabis sequences included 12 unique TEF1 haplotypes. These were phylogenetically diverse, suggesting that pathogenicity to C. sativa may have evolved independently within the F. oxysporum complex. Pathogenicity tests of the Israeli strains were confirmed by Koch's postulates assays. Strains of the five different f. sp. cannabis TEF1 haplotypes all caused wilt in cannabis seedlings but with varying levels of aggressiveness. The same isolates that originated from asymptomatic infected mother plants were found in wilted cuttings indicating that the pathogen can be spread via propagation material.

Scientific Publication
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