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Massive crossover suppression by CRISPR–Cas-mediated plant chromosome engineering
Year:
2022
Source of publication :
Nature Plants
Authors :
Lieberman, Michal
;
.
Volume :
Co-Authors:
  • Michelle Rönspies, 
  • Carla Schmidt, 
  • Patrick Schindele, 
  • Michal Lieberman-Lazarovich, 
  • Andreas Houben  
  • Holger Puchta 
Facilitators :
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0
To page:
0
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Total pages:
1
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Abstract:

Recent studies have demonstrated that not only genes but also entire chromosomes can be engineered using clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPER-associated protein 9 (Cas9)1,2,3,4,5. A major objective of applying chromosome restructuring in plant breeding is the manipulation of genetic exchange6. Here we show that meiotic recombination can be suppressed in nearly the entire chromosome using chromosome restructuring. We were able to induce a heritable inversion of a >17 Mb-long chromosome fragment that contained the centromere and covered most of chromosome 2 of the Arabidopsis ecotype Col-0. Only the 2 and 0.5 Mb-long telomeric ends remained in their original orientation. In single-nucleotide polymorphism marker analysis of the offspring of crosses with the ecotype Ler-1, we detected a massive reduction of crossovers within the inverted chromosome region, coupled with a shift of crossovers to the telomeric ends. The few genetic exchanges detected within the inversion all originated from double crossovers. This not only indicates that heritable genetic exchange can occur by interstitial chromosome pairing, but also that it is restricted to the production of viable progeny.

Note:
Related Files :
genetic engineering
transgenic plants
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More details
DOI :
10.1038/s41477-022-01238-3
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
62425
Last updated date:
02/11/2022 14:26
Creation date:
02/11/2022 14:26
Scientific Publication
Massive crossover suppression by CRISPR–Cas-mediated plant chromosome engineering
  • Michelle Rönspies, 
  • Carla Schmidt, 
  • Patrick Schindele, 
  • Michal Lieberman-Lazarovich, 
  • Andreas Houben  
  • Holger Puchta 
Massive crossover suppression by CRISPR–Cas-mediated plant chromosome engineering

Recent studies have demonstrated that not only genes but also entire chromosomes can be engineered using clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPER-associated protein 9 (Cas9)1,2,3,4,5. A major objective of applying chromosome restructuring in plant breeding is the manipulation of genetic exchange6. Here we show that meiotic recombination can be suppressed in nearly the entire chromosome using chromosome restructuring. We were able to induce a heritable inversion of a >17 Mb-long chromosome fragment that contained the centromere and covered most of chromosome 2 of the Arabidopsis ecotype Col-0. Only the 2 and 0.5 Mb-long telomeric ends remained in their original orientation. In single-nucleotide polymorphism marker analysis of the offspring of crosses with the ecotype Ler-1, we detected a massive reduction of crossovers within the inverted chromosome region, coupled with a shift of crossovers to the telomeric ends. The few genetic exchanges detected within the inversion all originated from double crossovers. This not only indicates that heritable genetic exchange can occur by interstitial chromosome pairing, but also that it is restricted to the production of viable progeny.

Scientific Publication
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