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SERS-based immunosensor for E. coli contaminants detection in milk using silver-coated nanoporous silicon substrates
Year:
2023
Source of publication :
Talanta
Authors :
Shtenberg, Giorgi
;
.
Volume :
254
Co-Authors:

Divagar Muthukumar
Giorgi Shtenberg

Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:

The dairy sector is frequently affected by contagious and environmental factors that spread between animals by numerous means and induce the inflammatory disease of bovine mastitis (BM). Herein, silver decorated porous silicon (Ag-pSi) SERS platform was designed for rapid and reliable Escherichia coli (predominant BM pathogen) detection in various milk origins. The inherent surface void and pore morphology were physically optimized to augment the SERS effect using 4-aminothiphenol (4ATP) while achieving an enhancement factor >4.6 × 107. An indirect immunoassay evaluated the residual unreacted antibodies using an optimized 4ATP/Ag-pSi SERS platform modified with secondary antibodies. Under optimized conditions, the porous substrate offered high sensitivity toward target bacteria detection of 3 CFU mL−1 and linear response of 101–105 CFU mL−1. Moreover, the selectivity and specificity of the designed sensing platform were cross-validated against other interfering bacteria without compromising its performance efficiencies. Finally, the applicability of the developed system for real-life conditions was elucidated in different milk samples (bovine, goat, sheep) with recovery values of 78–115% compared to the conventional culture technique. Considering the complex media analysis, the miniaturized SERS platform is highly reliable, rapid and accurate that could be applicable for routine on-site analysis of various emerging pathogens relevant to BM management.

Note:
Related Files :
Escherichia coli
milk
pathogen detection
Porous silicon
SERS
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More details
DOI :
10.1016/j.talanta.2022.124132
Article number:
124132
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
63146
Last updated date:
09/01/2023 16:57
Creation date:
09/01/2023 16:56
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Scientific Publication
SERS-based immunosensor for E. coli contaminants detection in milk using silver-coated nanoporous silicon substrates
254

Divagar Muthukumar
Giorgi Shtenberg

SERS-based immunosensor for E. coli contaminants detection in milk using silver-coated nanoporous silicon substrates

The dairy sector is frequently affected by contagious and environmental factors that spread between animals by numerous means and induce the inflammatory disease of bovine mastitis (BM). Herein, silver decorated porous silicon (Ag-pSi) SERS platform was designed for rapid and reliable Escherichia coli (predominant BM pathogen) detection in various milk origins. The inherent surface void and pore morphology were physically optimized to augment the SERS effect using 4-aminothiphenol (4ATP) while achieving an enhancement factor >4.6 × 107. An indirect immunoassay evaluated the residual unreacted antibodies using an optimized 4ATP/Ag-pSi SERS platform modified with secondary antibodies. Under optimized conditions, the porous substrate offered high sensitivity toward target bacteria detection of 3 CFU mL−1 and linear response of 101–105 CFU mL−1. Moreover, the selectivity and specificity of the designed sensing platform were cross-validated against other interfering bacteria without compromising its performance efficiencies. Finally, the applicability of the developed system for real-life conditions was elucidated in different milk samples (bovine, goat, sheep) with recovery values of 78–115% compared to the conventional culture technique. Considering the complex media analysis, the miniaturized SERS platform is highly reliable, rapid and accurate that could be applicable for routine on-site analysis of various emerging pathogens relevant to BM management.

Scientific Publication
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