תפריט נגישות
ניגודיות עדינהניגודיות גבוהה
מונוכרום
הדגשת קישורים
חסימת אנימציה
פונט קריא
סגור
איפוס הגדרות נגישות
להורדת מודול נגישות חינם תפריט נגישותניגודיות עדינהניגודיות גבוההמונוכרוםהדגשת קישוריםחסימת אנימציהפונט קריאסגוראיפוס הגדרות נגישותלהורדת מודול נגישות חינםYinghui Li
Zhen-Zhen Wei
Hanan Sela
Liubov Govta
Valentyna Klymiuk
Rajib Roychowdhury
Harmeet Singh Chawla
Jennifer Ens
Krystalee Wiebe
Valeria Bocharova
Roi Ben-David
Prerna B Pawar
Yuqi Zhang
Samidha Jaiwar
István Molnár
Jaroslav Doležel
Gitta Coaker
Curtis J Pozniak
Tzion Fahima
Gene cloning in repeat-rich polyploid genomes remains challenging. Here we describe a strategy for overcoming major bottlenecks in the cloning of the powdery mildew (Pm) resistance gene (R-gene) Pm69 derived from tetraploid wild emmer wheat (WEW). A conventional positional cloning approach was not effective due to suppressed recombination. Chromosome sorting was compromised by insufficient purity. A Pm69 physical map, constructed by assembling Oxford Nanopore Technology (ONT) long-read genome sequences, revealed a rapidly evolving nucleotide-binding leucine-rich repeat (NLR) R-gene cluster with structural variations. A single candidate NLR was identified by anchoring RNASeq reads of susceptible mutants to ONT contigs and was validated by virus-induced gene silencing. Pm69 is likely a newly evolved NLR, which was discovered only in one location across the WEW distribution range in Israel. Pm69 was successfully introgressed into cultivated wheat, and a diagnostic molecular marker was used to accelerate its deployment and pyramiding with other R-genes.
Yinghui Li
Zhen-Zhen Wei
Hanan Sela
Liubov Govta
Valentyna Klymiuk
Rajib Roychowdhury
Harmeet Singh Chawla
Jennifer Ens
Krystalee Wiebe
Valeria Bocharova
Roi Ben-David
Prerna B Pawar
Yuqi Zhang
Samidha Jaiwar
István Molnár
Jaroslav Doležel
Gitta Coaker
Curtis J Pozniak
Tzion Fahima
Gene cloning in repeat-rich polyploid genomes remains challenging. Here we describe a strategy for overcoming major bottlenecks in the cloning of the powdery mildew (Pm) resistance gene (R-gene) Pm69 derived from tetraploid wild emmer wheat (WEW). A conventional positional cloning approach was not effective due to suppressed recombination. Chromosome sorting was compromised by insufficient purity. A Pm69 physical map, constructed by assembling Oxford Nanopore Technology (ONT) long-read genome sequences, revealed a rapidly evolving nucleotide-binding leucine-rich repeat (NLR) R-gene cluster with structural variations. A single candidate NLR was identified by anchoring RNASeq reads of susceptible mutants to ONT contigs and was validated by virus-induced gene silencing. Pm69 is likely a newly evolved NLR, which was discovered only in one location across the WEW distribution range in Israel. Pm69 was successfully introgressed into cultivated wheat, and a diagnostic molecular marker was used to accelerate its deployment and pyramiding with other R-genes.
