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Spermidine synthase is essential for vegetative growth, stress response, secondary metabolism and pathogenicity in Alternaria alternata
Year:
2023
Source of publication :
Postharvest Biology and Technology
Authors :
Prusky, Dov
;
.
Volume :
207
Co-Authors:

Jing Yuan
Yongxiang Liu
Yangyang Yang
Yongcai Li 
Miao Zhang
Xiaojing Wang
Yuanyuan Zong
Yang Bi
Dov B. Prusky 

Facilitators :
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Total pages:
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Abstract:

Black spot, caused by the fungus Alternaria alternata, is one of the postharvest diseases that cause significant economic losses. Polyamine metabolism in fungi plays key roles in cellular processes and pathogen-host interactions. However, little is known about their detailed regulatory mechanisms on pathogenicity of plant fungi. In this study, we characterized the spermidine synthase AaSPDS in A. alternata. Knocking out AaSPDS caused a total loss of vegetative growth and sporulation which was partially restored by exogenous spermidine (Spd), so supplying 0.5 mM exogenous Spd to medium for evaluating the characteristics of WT and ΔAaSPDS. The deletion strain showed albino colonies, sparse mycelial tips and significant decreased biomass accumulation and melanin production. ΔAaSPDS strain was also more sensitive to osmotic stress, oxidative stress and cell wall-perturbing agents. Interestingly, the expression level of AaSPDS was greatly up-regulated during infection structure differentiation stage of A. alternata, and appressorium formation of the AaSPDS deletion strain was completely blocked. In addition, deletion of the AaSPDS gene led to a significant reduction in pathogenicity to pear fruit, altenuene (ALT) mycotoxin accumulation and the activities of cellulase (CL), β-glucosidase (β-GC) and polygalacturonase (PG). The results presented in this study suggested that AaSPDS influences the pathogenicity of A. alternata by regulating infection structure formation, cell wall degrading enzyme (CWDE) activity and mycotoxin production.

Note:
Related Files :
Alternaria alternata
Cell wall degrading enzymes
infection structures
Spermidine synthase
virulence
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More details
DOI :
10.1016/j.postharvbio.2023.112612
Article number:
112612
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
66163
Last updated date:
29/10/2023 16:49
Creation date:
29/10/2023 15:15
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Scientific Publication
Spermidine synthase is essential for vegetative growth, stress response, secondary metabolism and pathogenicity in Alternaria alternata
207

Jing Yuan
Yongxiang Liu
Yangyang Yang
Yongcai Li 
Miao Zhang
Xiaojing Wang
Yuanyuan Zong
Yang Bi
Dov B. Prusky 

Spermidine synthase is essential for vegetative growth, stress response, secondary metabolism and pathogenicity in Alternaria alternata

Black spot, caused by the fungus Alternaria alternata, is one of the postharvest diseases that cause significant economic losses. Polyamine metabolism in fungi plays key roles in cellular processes and pathogen-host interactions. However, little is known about their detailed regulatory mechanisms on pathogenicity of plant fungi. In this study, we characterized the spermidine synthase AaSPDS in A. alternata. Knocking out AaSPDS caused a total loss of vegetative growth and sporulation which was partially restored by exogenous spermidine (Spd), so supplying 0.5 mM exogenous Spd to medium for evaluating the characteristics of WT and ΔAaSPDS. The deletion strain showed albino colonies, sparse mycelial tips and significant decreased biomass accumulation and melanin production. ΔAaSPDS strain was also more sensitive to osmotic stress, oxidative stress and cell wall-perturbing agents. Interestingly, the expression level of AaSPDS was greatly up-regulated during infection structure differentiation stage of A. alternata, and appressorium formation of the AaSPDS deletion strain was completely blocked. In addition, deletion of the AaSPDS gene led to a significant reduction in pathogenicity to pear fruit, altenuene (ALT) mycotoxin accumulation and the activities of cellulase (CL), β-glucosidase (β-GC) and polygalacturonase (PG). The results presented in this study suggested that AaSPDS influences the pathogenicity of A. alternata by regulating infection structure formation, cell wall degrading enzyme (CWDE) activity and mycotoxin production.

Scientific Publication
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