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פותח על ידי קלירמאש פתרונות בע"מ -
Expression of the Health-Beneficial Stilbenes in Transgenic 'Spadona' Pear (Pyrus communis)
Year:
2005
Source of publication :
Acta Horticulturae
Authors :
סיון, ליאור
;
.
פליישמן, משה
;
.
שליזרמן, לודמילה
;
.
Volume :
671
Co-Authors:
Flaishman, M.A., Department of Fruit Trees, Institute of Horticulture, ARO, Volcani Center, P.O. Box 6, Bet Dagan, Israel
Shlizerman, L., Department of Fruit Trees, Institute of Horticulture, ARO, Volcani Center, P.O. Box 6, Bet Dagan, Israel
Cohen, Y., Department of Fruit Trees, Institute of Horticulture, ARO, Volcani Center, P.O. Box 6, Bet Dagan, Israel
Kerem, Z., Institute of Biochemistry, Food Science and Nutrition, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Sivan, L., Institute of Biochemistry, Food Science and Nutrition, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Facilitators :
From page:
283
To page:
288
(
Total pages:
6
)
Abstract:
The gene encoding stilbene synthase is responsible for the synthesis of the compound resveratrol. Resveratrol and its glycosides are considered to have beneficial effects on human health, including anti-ageing, anti-inflammatory, antiplatelet and anti-carcinogenic activities. To introduce health-beneficial compounds into 'Spadona', a low chill requiring pear cultivar adapted to hot climates, leaf discs were transformed with grapevine (Vitis vinifera L.) cDNA encoding stilbene synthase, transcriptionally regulated by an enhanced cauliflower mosaic virus (CaMV) 35S promoter. Six transgenic 'Spadona' plants were obtained from 276 infected explants, corresponding to a transformation efficiency of 2.2%. Transgenic plants carrying the stilbene synthase gene were identified via polymerase chain reaction and Southern blot. Stilbene production was monitored in one-year-old rooted transgenic plants. The transgenic plants accumulated several new compounds not present in the non-transformed pear that were identified as stilbenes by highperformance liquid chromatography analysis. The major stilbenes were piceid and resveratroloside. The production of stilbene glucosides by an edible fruit may enhance its functionality as a health-promoting food. The functionality of the resveratrol analogs in the transgenic pear is enhanced by the combination of high concentrations in the transformed tissues and the fulfillment of the mouth-retention requirement due to chewing.
Note:
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DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר מתוך כינוס
;
.
Language:
אנגלית
Editors' remarks:
ID:
20364
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:35
You may also be interested in
Scientific Publication
Expression of the Health-Beneficial Stilbenes in Transgenic 'Spadona' Pear (Pyrus communis)
671
Flaishman, M.A., Department of Fruit Trees, Institute of Horticulture, ARO, Volcani Center, P.O. Box 6, Bet Dagan, Israel
Shlizerman, L., Department of Fruit Trees, Institute of Horticulture, ARO, Volcani Center, P.O. Box 6, Bet Dagan, Israel
Cohen, Y., Department of Fruit Trees, Institute of Horticulture, ARO, Volcani Center, P.O. Box 6, Bet Dagan, Israel
Kerem, Z., Institute of Biochemistry, Food Science and Nutrition, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Sivan, L., Institute of Biochemistry, Food Science and Nutrition, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Expression of the Health-Beneficial Stilbenes in Transgenic 'Spadona' Pear (Pyrus communis)
The gene encoding stilbene synthase is responsible for the synthesis of the compound resveratrol. Resveratrol and its glycosides are considered to have beneficial effects on human health, including anti-ageing, anti-inflammatory, antiplatelet and anti-carcinogenic activities. To introduce health-beneficial compounds into 'Spadona', a low chill requiring pear cultivar adapted to hot climates, leaf discs were transformed with grapevine (Vitis vinifera L.) cDNA encoding stilbene synthase, transcriptionally regulated by an enhanced cauliflower mosaic virus (CaMV) 35S promoter. Six transgenic 'Spadona' plants were obtained from 276 infected explants, corresponding to a transformation efficiency of 2.2%. Transgenic plants carrying the stilbene synthase gene were identified via polymerase chain reaction and Southern blot. Stilbene production was monitored in one-year-old rooted transgenic plants. The transgenic plants accumulated several new compounds not present in the non-transformed pear that were identified as stilbenes by highperformance liquid chromatography analysis. The major stilbenes were piceid and resveratroloside. The production of stilbene glucosides by an edible fruit may enhance its functionality as a health-promoting food. The functionality of the resveratrol analogs in the transgenic pear is enhanced by the combination of high concentrations in the transformed tissues and the fulfillment of the mouth-retention requirement due to chewing.
Scientific Publication
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